Abstract：To further understand the prevalence of swine influenza in pig farm in Henan province, 360 porcine nasal swabs and 35 swine lung and spleen samples of pigs that showed clinic signs of respiratory symptoms were collected from one big slaughterhouse and one clinic service of swine diseases in Henan Province. The chicken embryo inoculation method was used for virus isolation, the biological characterizations of swine influenza virus were tested and analysed. The results showed that three strains of swine influenza virus were isolated successfully, two isolates were H1N1 subtype and one was H1N2 subtype, named as: A/Swine/Henan/01/2012(H1N2), A/Swine/Henan/01/2012(H1N1) and A/Swine/Henan/02/2012(H1N1) respectively. On this basis, these strains isolated were purified by limited dilution cloning. Biological characteristics research showed: the isolates had hemagglutinin to 1% red cells of chicken, rabbit, mouse and bovine respectively. In addition, the isolates were sensitive to the chemical reagent of Ethyl ether, chloroform and acids of pH3.0. And the different strains of EID50 and ELD50 were different biological characteristics with each other, infection of mice were ill after the onset of 4 days. The swine influenza is a epidemic disease in Henan, and zoonotic subtype exist in pig farm, the influence to public health need to take more attention.
2013, Vol. 29 
