Abstract：ABSTRCT: In order to evaluate the role of Mac-1 on murine macrophage in recognition of Candida albicans. The model for the phagocytosis of Candida albicans inactivated yeast cells and live yeast cells by mouse macrophage cell line RAW264.7 was established, the Mac-1 mRNA expression was detected by RT-PCR, Mac-1 protein expression was detected by western blot, phagocytosis rate was detected by flow cytometry, culture cytokine levels was detected by ELISA. The siRNAs targeting interfere with Mac-1 on macrophage, targeting down the expression of Mac-1, phagocytosis rate and cytokine levels were detected. It was found that the Mac-1 mRNA and protein expression and cytokine secretion was no significant difference after RAW264.7 cells co-cultured with inactivated yeast cells and live yeast cells (P>0.05), the phagocytosis rate was 85.28% and 84.90% (P>0.05). After co-culture, IL-2, IFN-γ and other Th1-type crtokines, IL-4, IL-10 and other Th2-type cytokines showed different degrees of rise. Mac-1 on RAW264.7 cells was interferenced by siRNA, then co-cultured with Candida albicans, phagocytic rate decreased to 28.60% (P<0.05). Meanwhile, the macrophages secrete cytokines decreased. The results indicate that in early stage of innate immunity, Mac-1 on macrophage is one of the pattern recognition receptors that participated recognition and mediated phagocytosis of Candida albicans. Th1-type cytokines of IL-2, IFN-gamma, Th2-type cytokines of IL-4, IL-10 are involved in anti-infection immunity of macrophage resistance to Candida albicans.
2013, Vol. 29 
