Abstract:We applied real-time PCR to study the endocytic pathway of JEV entry into BHK-21 cells. BHK-21 cells were treated with Chlorpromazine, Nystatin and Cytochalasin D to block clathrin-mediated endocytosis, caveolae-mediated endocytosis, and phagocytosis, respectively. Then those treated cells were infected with JEV. Real-time RT-PCR assay was used in this study to estimate the intracellular virus copy-number at 1 h, 12 h, and 24 h after infection, respectively. Compared with control group, the intracellular virus copy numbers of three groups treated with drug were in different degrees of decline. The change showed in the group treated with Chlorpromazine was the most obvious, and the intracellular virus copy number decreased by 59.58%, 70.20% and 61.35%, respectively; the intracellular virus copy number in the cytochalasin D group decreased by 23.59%, 55.79% and 30.14%, and in the nystatin group decreased by 35.56%, 58.10% and 34.40%, respectively. Results indicated that JEV infect BHK-21 cells by clathrin-mediated endocytosis.
曹三杰 刘凯. JEV入侵BHK-21细胞内吞途径的初步研究[J]. 中国人兽共患病学报, 2013, 29(8): 766-770.
san-jie cao. preliminary research of the endocytic pathway of JEV entry into BHK-21 cell. Chinese Journal of Zoonoses, 2013, 29(8): 766-770.