Identification on interaction of C terminal of microneme protein 6 with aldolase in Toxoplasma gondii
ZHENG Bin1,YIN Zhi-kui2,ZHAN Xi-mei3
1.Department of Parasitology, Xinxiang Medical University, Xinxiang 453000, China; 2.Department of Pharmacology, Xinxiang Medical University, Xinxiang 453000, China; 3.Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510089, China
Abstract：This study aims to identify the interaction of C terminal of microneme protein 6 (MIC6C) with aldolase in Toxoplasma gondii and to observe the proteins location in tachyzoites of T. gondii. Anti-GST-MIC6C or preimmune rabbit sera were added to protein G coated sepharose beads, and then parasite lysate was added to the beads and incubated. Beads were washed, and the protein-coated beads were dissolved in protein loading buffer. Proteins were resolved by SDS-PAGE and transferred to NC membranes for immunoblotting. Protein bands were found in co-sediment products coming from immunoprecipitation (IP) experiment of anti-GST-MIC6C antibody, compared with the control (preimmune rabbit sera). MIC6 and aldolase, examining by immunofluorescence (IF) microscopy of Toxoplasma tachyzoites smears, were apically localized. Its suggested that the MIC6C interacts with aldolase, and co-localizes in T. gondii.
郑斌,尹志奎,詹希美. 弓形虫MIC6羧基端与醛缩酶相互作用的鉴定[J]. 中国人兽共患病学报, 2013, 29(9): 883-885.
ZHENG Bin,YIN Zhi-kui,ZHAN Xi-mei. Identification on interaction of C terminal of microneme protein 6 with aldolase in Toxoplasma gondii. Chinese Journal of Zoonoses, 2013, 29(9): 883-885.
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2013, Vol. 29 
