Abstract：In this study, we explore the killing effect, the expression of caspase-3 in the cells, and the changes in the microscopic structure of Echinococcus protoscoleces after extracorporeal Gamma-ray irradiation. The Echinococcus protoscoleces were treated with different doses of Gamma-ray irradiation. On the basis of in vitro culture, the protease activity of caspase-3 within various groups of protoscoleces cells were tested after the irradiation, the death incidence of protoscoleces were observed, and dynamic observation were conducted for the changes in the morphology and structure of protoscoleces under inverted microscope. It was found that after gamma-ray irradiation, the death incidence of protoscoleces were increased, and the expression of caspase-3 was enhanced; meanwhile, phasal changes of swelling, collapse and breaking-up appeared in the morphosis and structure of protoscoleces. The results indicate that extracorporeal Gamma-ray irradiation could directly disinfect protoscoleces and induce the apoptosis of protoscoleces cells.
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2013, Vol. 29 
