Helicobacter pylori effect of antibacterial peptidesfrom Musca domestica larvae
HUANG Jian1,MO Fei2
1.Biochemistry Department, the Affiliated Hospital of Guiyang Medical University, Guiyang 550004, China; 2.Department of Clinical Laboratory, Guiyang Medical University, Guiyang 550004, China
Abstract:This paper aims to discuss the antibacterial mechanism of antibacterial peptides for Musca domestica on Helicobacter pylori. The mechanism for action of Musca domestica on Helicobacter pylori would be discussed preliminarily in this paper by means of the methods and techniques of drawing curves of bacteriostasis, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and two-dimensional electrophoresis (2-DE). The antibacterial peptides of Musca domestica larva with the concentration of 40 μg/mL made Hp unable to achieve the normal growth peak and come to the log phase. Instead, it came to the decline phase directly after 1 day. It was detected by SDS-PAGE that, the concentration of protein bands with the molecular weight of about 66.4 kDa of the experimental group decreased compared with the concentration of protein bands of the control group. But the concentration of protein bands with the molecular weight of about 40 kDa of the experimental group increased compared with the concentration of protein bands of the control group. The result of 2-DE showed that the antibacterial peptides of Musca domestica larva interfered the antioxidant system of Helicobacter pylori and influenced the expression of functional protein with energy metabolism and stress effect of Helicobacter pylori. It came to a conclusion that the antibacterial peptides of Musca domestica larva have significant inhibitory effect on Helicobacter pylori. The antibacterial mechanism is brought into play by inhibiting the growth of Helicobacter pylori, disturbing its antioxidant system and influencing its energy metabolism and other aspects.
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