Abstract:Molecular diagnostics methods were used for detecting Bacillus anthraci and identifying virulence genes directly from clinical material and infected beef in a cutaneous anthrax outbreak in Lianyungang City, Jiangsu Province, PR China, 2012. Blood and eschar samples were obtained from suspected cutaneous anthrax patients and the beef was collected from the sick cow. The chromosome gene (rpoB) and plasmid genes (pag, capA) of B. anthraci were detected directly from DNA of samples by real-time PCR. The virulence genes (cya, pag, lef and cap) of B. anthraci were identified by conventional PCR and geneticly analyzed by sequencing. The results showed that the identity of DNA from patients eschar and beef were further confirmed positive to the genes rpoB, pag and capA of B. anthraci. The sequencing results confirmed that the amplified sequences were virulence genes of B. anthraci. The sequences of each virulent gene from patients and beef were identical. The results revealed that this outbreak was caused by virulent strain of B. anthracis transmitted by contacts of the beef of the sick cow.
谈忠鸣,张忠献,顾 玲,胡建利,朱叶飞,祁 贤,董 晨,汤奋扬,周明浩,鲍倡俊. 一起皮肤炭疽疫情病原的实验室检测及毒力基因鉴定[J]. 中国人兽共患病学报, 2013, 29(10): 996-999.
TAN Zhong-ming,ZHANG Zhong-xian,GU Ling,HU Jiang-li,ZHU Ye-fei, QI Xian,DONG Chen,TANG Feng-yang,ZHOU Ming-hao,BAO Chang-jun. Detection of Bacillus anthracis and identification of virulence genes directly from clinical material and beef in an anthrax outbreak. Chinese Journal of Zoonoses, 2013, 29(10): 996-999.
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