Mosquito-borne arbovirus investigation in Yunfu Port, China
LUO Lin1, 2, LIU Chuan-ge1, 3, HU Long-fei1, 4, ZHENG Kui5, DENG Ji-tang1, MA Ze-zheng1, ZHANG You1, YAO Jing-yu4, GU Da-yong4
1. Department of Health Quarantine, Yunfu Entry-exit Inspection and Quarantine Bureau, Yunfu 527300, China; 2. Department of Microbiological Examination, Nanhai District Center for Disease Control and Prevention, Foshan 528200, China; 3.Department of Epidemiology, Yunfu Municipal Center for Disease Control and Prevention, Yunfu 527300, China; 4.Institute of Disease Control and Prevention, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen 518045, China; 5.Health Quarantine Laboratory, Guangdong Inspection and Quarantine Technology Center, Guangzhou 510700, China)
Abstract：Mosquito samples were collected at Yunfu Port in China and detected for representative mosquito-borne arboviruses during May to November of 2011. Representative mosquito-borne arboviruses, including dengue, Japanese encephalitis (JE), chikungunya, West Nile, and yellow fever viruses, were detected by multiple real-time fluorescence PCR assay. A further study of PCR amplification and sequence analysis to the positive samples were performed. A total of 39 254 individual mosquitoes were collected from 460 mosquito pools at Yunfu Port. All the virus including dengue, chikungunya, West Nile, and yellow fever viruses showed negative results except for 14 positive results with JE virus (JEV) nucleic acid detected by real-time PCR assay. The genotype of JEV was confirmed as genotype Ⅰ by PCR amplification and sequence analysis of E gene. The genotype Ⅰ JEV carried by mosquito was found for the first time in Guangdong. The minimum infection rate (MIR) of JEV in Culex tritaeniorhynchus was 0.937‰, which was slightly higher than that in Anopheles sinensis at 0.385‰ at Yunfu Port. Although the difference in MIR was not statistically significant, the outbreak and epidemic risk of mosquito-borne arboviral disease still exists. The primary investigation of mosquito-borne arbovirus at Yunfu Port would be useful to offer an effective method for future mosquito-borne arbovirus surveillance and detection.
罗琳,刘传鸽,胡龙飞,郑夔,邓继棠,马泽征,张友,姚旌羽,顾大勇. 中国云浮口岸蚊媒病毒的首次调查[J]. 中国人兽共患病学报, 2013, 29(11): 1044-1049.
LUO Lin, LIU Chuan-ge, HU Long-fei, ZHENG Kui, DENG Ji-tang, MA Ze-zheng, ZHANG You, YAO Jing-yu, GU Da-yong. Mosquito-borne arbovirus investigation in Yunfu Port, China. Chinese Journal of Zoonoses, 2013, 29(11): 1044-1049.
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