Molecular characterization of a porcine kobuvirus strain isolated in Fujian, China
XIU Jin-sheng1, HU Chong-wei1, CHEN Ru-jing2, WANG Bin1, LI Tao1, CHEN Qiu-yong1, LIN Qun-qun1
1.College of Animal Science, Fujian Agriculture and Forestry University, Fujian 350002, China; 2.Institute of Animal Husbandry and Veterinary Medicine/Fujian Animal Diseases Control Technology Development Center, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Abstract：The objective is to understand the complete genome and the genome organization of the porcine kobuvirus isolated in Fujian (PKV-FJ), China. Reverse transcription polymerase chain reaction were used to amplified the target gene fragments of the PKV-FJ, and the rapid-amplification of cDNA ends methods were employed to obtained the terminals for the virus using the 5′/3′ RACE Kit (2nd generation). The full-genome of the PKV-FJ was 8 210 bp including a single open reading frame (ORF) of 7 467 bp encoding a polyprotein with 2 488 amino acid. The 5′ untranslated regions was 576 bp long, while the 3′ untranslated regions was 167 bp long. The polyprotein nucleotide sequences shared the highest identities with CH/HNXX-4/2012 at 89.2%, and the lowest with swine/S-1-HUN/2007/Hungary at 87.6%, respectively.
修金生,胡崇伟,陈如敬,王斌,李涛,陈秋勇,林群群. 猪嵴病毒福建株全基因克隆及序列分析[J]. 中国人兽共患病学报, 2013, 29(11): 1076-1079.
XIU Jin-sheng, HU Chong-wei, CHEN Ru-jing, WANG Bin, LI Tao, CHEN Qiu-yong, LIN Qun-qun. Molecular characterization of a porcine kobuvirus strain isolated in Fujian, China. Chinese Journal of Zoonoses, 2013, 29(11): 1076-1079.
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2013, Vol. 29 
