Sequence analysis on kinetoplast DNA minicircles of Chinese Leishmania isolates in different foci
DING Dan1, GAO Chun-hua1, YANG Yue-tao1, ZHANG Song2, WANG Jun-yun1
1.National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention/ Laboratory of Parasite and Vector Biology, Ministry of Health / WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China; 2.Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi 830002, China
Abstract:This study conducted an analysis for the sequence heterogeneity of the kinetoplast DNA minicircles in Chinese Leishmania isolates in different foci. Kinetoplast DNA minicircles were amplified using a set of primers from conserved region of minicircle of Leishmania. The PCR products were then cloned into pGEM-T vector and 5 positive clones were selected to be sequenced randomly. Sequence alignment was done by DNAStar software and phylogenic tree was constructed using MEGA software. Results revealed that single minicircles were amplified from Leishmania strains SC, KXG-Xu, KXG-65, JIASHI-1, and 801 from different foci of China using primer KP1-KP2, with the length of 858 bp, 858 bp, 858 bp, 750 bp, and 748 bp, respectively. The sequence identity among SC, KXG-Xu and KXG-65 were over 99%, and JIASHI-1 and 801 both showed high heterogeneity with other strains of Leishmania. Strain 801 was close to the isolates of Leishmania donovani, and the strain JIASHI-1 was close to the isolate of viscerotropic Leishmania. SC, KXG-Xu and KXG-65 were also clustered together, while they were closer to viscerotropic Leishmania than to cutaneotropic Leishmania. It showed that sequence variations exist in kinetoplast minicircles of Leishmania from different foci of China.
丁丹,高春花,杨玥涛,张松,汪俊云. 我国不同流行区利什曼原虫分离株动基体小环DNA序列分析[J]. 中国人兽共患病学报, 2013, 29(11): 1055-1059.
DING Dan, GAO Chun-hua, YANG Yue-tao, ZHANG Song, WANG Jun-yun. Sequence analysis on kinetoplast DNA minicircles of Chinese Leishmania isolates in different foci. Chinese Journal of Zoonoses, 2013, 29(11): 1055-1059.
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