Analysis of the hemagglutinin gene characteristics of H3N2 subtype influenza viruses isolated in Fujian, 2010-2012
XIE Jian-feng1, SHEN Xiao-na1, WANG Mei-ai1, YANG Shi-qin1, XIU Wen-qiong1, HUANG Meng1, ZHANG Yan-hua1, WENG Yu-wei1, YAN Yan-sheng1, ZHENG Kui-cheng1, 2
1.Fujian Key Laboratory for Zoonoses Research, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China; 2. School of Public Health, Fujian Medical University, Fuzhou 350004, China
Abstract:In order to explore the characteristics of hemagglutinin 1 (HA1) gene of H3N2 subtype influenza in Fujian, 28 strains were randomly selected for detection during 2010 to 2012. After the RNA from isolated strains was extracted, fragments of HA1 genes were amplified by RT-PCR and sequenced. Our results on genetic evolution indicated that there was a homology of 96.4%-100% among the HA1 nucleotide sequence of 28 H3N2 subtype influenza viruses, and the sequence variation rates were proportional to time. A total of 43 sites in amino acid variation was identified in the HA1 region, resulting 10 variations scattered in four antigen determinants and 7 variations occurred near the receptor binding sites (RBS). Compared with vaccine strain (A/Perth/16/2009), there was an antigenic drift in HA1 region in 2012. In conclusion, the H3N2 subtype influenza between 2010 and 2012 from Fujian has a gradually antigenic variation and more attention should be paid to the surveillance of their mutation for control and prevention strategies.
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