Investigation of drug-resistance mechanism to rifampin in Mycobacterium abscessus
FAN Gui-rong, YANG Zhi-bang, HUANG Jin, GAO Ji-ye
Department of Pathobiology, College of Basic Medicine, Experimental Teaching Center of Basic Medicine / Pathogenic Biology and Immunology Laboratory, Chongqing Medical University, Chongqing 400016, China
Abstract:To study the drug-resistance mechanism of Mycobacterium abscessus to rifampin, the MICs of rifampin against Mycobacterium abscessus were determined by both microdilution checkerboard method with different concentrations of agents for changing cell wall permeability and efflux pump inhibitors, respectively. They were used to analyze the effects of cell wall permeability and active efflux on the mechanism of drug-resistance to rifampin in Mycobacterium abscessus. The MICs of rifampin against Mycobacterium abscessus were gradually decreased as the concentrations of the joined TWEEN-80, Trixon X-100, methylbenzene, aether, CCCP, reserpine, and pantoprazole increased, which were changed from 512 μg/mL to less than 32 μg/ mL for TWEEN-80, Trixon X-100, CCCP, and pantoprazole and from 512 μg/mL to 64 μg/ mL for methylbenzene, aether and reserpine. They were the optimal concentrations for 0.1% TWEEN-80, 0.00625% Trixon X-100, 0.1 μg/mL CCCP, 32 μg/mL pantoprazole, 0.25% methylbenzene, 0.25% aether, and 8 μg/mL reserpine to affect the antibacterial activity of rifampin against Mycobacterium abscessus. These findings indicated that the permeability of cell wall and the active efflux play important roles in the drug-resistance mechanism of Mycobacterium abscessus to rifampin.
范贵荣, 杨致邦, 黄进, 高继业. 脓肿分枝杆菌耐利福平机制的研究[J]. 中国人兽共患病学报, 2013, 29(12): 1172-1176.
FAN Gui-rong, YANG Zhi-bang, HUANG Jin, GAO Ji-ye. Investigation of drug-resistance mechanism to rifampin in Mycobacterium abscessus. Chinese Journal of Zoonoses, 2013, 29(12): 1172-1176.
[1] Talati NJ, Rouphael N, Kuppalli K, et al. Spectrum of CNS disease caused by rapidly growing mycobacteria[J]. Lancet Infect Dis, 2008, 8(6):390-398. DOI: 10.1016/S1473-3099(08)70127-0 [2]Furuya EY, Paez A, Srinivasan A, et al. Outbreak of Mycobacterium abscessus wound infections among lipotourists'from the United States who underwent abdominoplasty in the Dominican Republic[J]. Clin Infect Dis, 2008, 46(8):1181-1188. DOI: 10.1086/529191 [3]Zhibang Y, Bixia Z, Qishan L, et al. Large-scale outbreak of infection with Mycobacterium chelonae subsp. abscessus after penicillin injection[J]. J Clin Microbio1, 2002, 40(7): 2626-2628. DOI: 10.1128/JCM.40.7.2626-2628.2002 [4]Medjahed H, Gaillard JL, Reyrat JM. Mycobacterium abscessus: a new player in the mycobacterial field[J]. Trends Microbiol, 2010, 18(3): 117-123. DOI: 10.1016/j.tim.2009.12.007 [5]Ripoll F, Pasek S, Schenowitz C, et al. Non mycobacterial virulence genes in the genome of the emerging pathogen Mycobacterium abscessus[J]. PLoS One, 2009, 4(6): e5660. DOI: 10.1371/journal.pone.0005660 [6]Hofling-Lima AL, de Freitas D, Sampaio JL, et al. In vitro activity of fluoroquinolones against Mycobaterium abscessus and Mycobacterium chelonae causing infectious keratitis after LASIK in Brazil[J]. Cornea, 2005, 24(6): 730-734. [7]Zhang RF, Yang ZB. Advance in research of drug resistance molecular mechanism of Mycobaterium abscessus[J]. J Chongqing Med Univ, 2006, 31: 106-108. (in Chinese) 张任飞,杨致邦.脓肿分枝杆菌的耐药分子机制研究进展[J].重庆医科大学学报,2006,31: 106-108. [8]Sha W, Weng XH, Xiao HP, et al. Investigation of drug-resistance to rifampin and rpoB gene sequence analysis of Mycobaterium abscessus[J]. Chin J Tuberc Respir Dis, 2003, 26(9): 352-356. (in Chinese) 沙魏,翁心华,肖和平,等.脓肿分枝杆菌耐利福平及rpoB基因突变情况的研究[J].中华结核和呼吸杂志, 2003, 26(9): 352-356. [9]Xu YP, Cao JR, Li LZ, et al. Combination antimicrobial susceptibility test on 43 multi-drug-resistant isolates of Pseudomonas aeruginosa[J]. Chin J Nosocomiol, 2007, 17(2): 210-212. (in Chinese) 徐雅萍,曹敬荣,李连赞,等.多重耐药铜绿假单胞菌体外联合药敏研究[J].中华医院感染学杂志, 2007, 17(2) : 210-212. [10]Sha W, Weng XH, Xiao HP, et al. Investigation of the membrane permeability of Mycobaterium abscessus[J]. Chin J Antituberculosis, 2005, 27(6): 353-357. (in Chinese) 沙魏,翁心华,肖和平,等.脓肿分枝杆菌细胞壁通透性的研究[J].中国防痨杂志,2005,27(6):353-357. [11]Marchand I, Damier-Piole L, Courvalin P, et al. Expression RND-type efflux pump AdeABC in Acinetobacter baumanni regulated by the adeRS two-component system[J]. Antimicrob Agents Chemother, 2004, 48(9):3298-3304. DOI: 10.1128%2FAAC.48.9.3298-3304.2004 [12]Buckley AM, Webber MA, Cooles S, et al. The AcrAB-TolC efflux system of Salmonella enterica serovar Typhimurium plays a role in pathogenesis[J]. Cell Microbiol, 2006, 8(5):847-856. DOI: 10.1111/j.1462-5822.2005.00671.x [13]Chau SL, Chu YW, Houang ET. Novel resistance-nodulation-c division efflux system AdeDE in Acinetobacter genomic DNA group 3[J]. Antimicrob Agents Chemother, 2004, 48(10):4054-4055. DOI: 10.1128/AAC.48.10.4054-4055.2004 [14]Zhang Z, Liu ZQ, Zheng PY, et al. Effects of efflux pump inhibitors on the multidrug resistance of Helicobacter pylori[J]. World J Gastroenterol, 2010, 16(10): 1279-1284. DOI: 10.3748/wjg.v16.i10.1279 [15]Ribera A, Ruiz J, Jiminez de Anta MT, et al. Effect of an efflux pump inhibitor on the MIC of nalidixic acid for Acinetobacter baumannii and Stenotrophomonas maltophilia clinical isolates[J]. J Antimicrob Chemother, 2002, 49(4): 697-698. DOI: 10.1093/jac/49.4.697