Abstract:To evaluate the value of rSjSDISP on the diagnosis of schistosomiasis japonica by fast ELISA, the recombinant plasmid pET32a/rSjSDISP was constructed, induced and purified. For comparing the value of early diagnosis on schistosomiasis japonica, we used methods of rSjSDISP F-ELISA and SEA-IHA to detect serum of rabbit in different period of infection with Schistosoma japonicum. By detecting the serum from 31 patients of parasitic diseases (18 with schistosomiasis, 9 with lung flukes, and 18 with liver fluke), we preliminary evaluated the diagnostic efficiency of the F-ELISA based on rSjSDISP antigen. By detecting 335 samples of human serum from the epidemic field of schistosomiasis japonica, we compared the sensitivity and specificity among methods of IHA, SEA F-ELISA and rSjSDISP F-ELISA. It was found that F-ELISA using rSjSDISP as coating antigen could distinguish the sera of rabbits infected with schistosomiasis after 28 days and there was no significantly statistical difference in the test results between rSjSDISP F-ELISA and SEA-IHA. And the sensitivity, specificity, and Youden ration of rSjSDISP F-ELISA method was 94.4, 93.0, and 0.88, respectively. There was no significantly statistical difference in the results among rSjSDISP in F-ELISA, SEA in F-ELISA, and SEA in IHA (P>0.05). It is evident that recombinant SjSDISP protein is a substitute for soluble egg antigen (SEA) as coating antigen in serodiagnosis of schistosomiasis. The rSjSDISP constructed by F-ELISA would have great potential in schistosomiasis diagnosis for its simplicity, stability, and easy standardization.
许进,汪世平,周云飞,金晶,彭交凤,尹铁球,何鑫,李芬,何军,徐绍锐. 日本血吸虫琥珀酸脱氢酶铁硫蛋白重组抗原的制备及其诊断价值的研究[J]. 中国人兽共患病学报, 2014, 30(2): 109-115.
XU Jin,WANG Shi-ping,ZHOU Yun-fei,JIN Jing,PENG Jiao-feng,YIN Tie-qiu,HE Xin,LI Fen, HE Jun,XU Shao-rui. Prokaryotic expression of succinate dehydrogenase iron-sulfur protein and evaluation of it on immunodiagnosis of schistosomiasis japonica. Chinese Journal of Zoonoses, 2014, 30(2): 109-115.
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