Abstract:To analyze the Envelope (E) gene of type 1, 2 dengue virus (DENV 1, DENV 2) isolated from Guangzhou in 2012 and to investigate the infection source and virus genotype, 478 serum samples were collected from 478 patients in acute phase of dengue fever. Dengue virus was cultured and isolated by C6/36 cells. Whole length E gene was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR), and then sequenced. The phylogenetic tree was drawn by neighbor-joining method. Both data on epidemiologic and molecular studies were processed and analysed. Six strains of DENV 1 and two strains of DENV 2 were isolated from samples of the 478 patients. The E gene sequence of these strains was obtained by sequencing. Both of DENV 1 and 2 E were 1 485 bp in length, encoding 495 amino acids. Nucleotide sequence homology of six strains DENV 1 was between 97.4%-99.9%, and the deduced amino acids sequence homology was between 99.2%-100.0%, whose strains’ homology was very close with DENV 1 virus epidemic strains in 2011, 2006 and 2004 in China. DENV 2 nucleotide sequence homology was 91.3%, and the deduced amino acids sequence homology was 97.8%, whose strains’ homology was relatively distant with DENV 2 virus epidemic strains in China. The phylogenetic analysis showed that DENV 1 belonged to one genotype (Asian) in common, and DENV 2 belonged to two genotypes (Malaysia/India subcontinent and Southeast Asia). It’s presumed that all strains of DENV 1 and 2 in Guangzhou in 2012 are imported. DENV 1 may originate from the Cambodia and Foshan, China. DENV 2 viruses may originate from the Bengal and Burma.
白志军,曹毅敏,曹庆,杨智聪,狄飚. 广州地区2012年1、2型登革病毒基因型特征研究[J]. 中国人兽共患病学报, 2014, 30(2): 207-212.
BAI Zhi-jun,CAO Yi-min,CAO Qing,YANG Zhi-cong,DI Biao. Genotyping of dengue virus type 1 and 2 strains isolated in Guangzhou area, 2012. Chinese Journal of Zoonoses, 2014, 30(2): 207-212.
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