Abstract:To explore genotyping on merozoite surface protein-1 of Plasmodium vivax before malaria elimination in Hainan Island, PCR amplification combined sequencing with alignment were conducted and phylogenetic tree was also constructed. In 27 cases, 24 cases belonged to Sal type, 2 cases were Belem type and 1 case was recombinant type. Sal type, which dominated type in those cases, was classified 9 sub-Sal types in 24 cases; two new continuous amino acid substitutions (DKKLLKEYELNADEKTKINQN) were found in two sub-Sal types, although individual amino acid substitution was found in other amino acid sequences. A new recombinant type was also found in those cases. Phylogenetic tree also showed Sal-1 type, belem type and recombined type belonged to different clusters. Sal-a and Sal-b subtypes belonged to one cluster and other subtypes belong to other clusters. Two case of belem type, characterized as poly Q, belonged to some belem type and the evidence from phylogenetic tree also supported it. The constitution of recombined type belonged to II/Q/S type and this type needed further study because of no homologous sequence in database. In Hainan Island, gene diversity in merozoite surface protein-1 of Plasmodium vivax manifested before malaria elimination and Sal-1 type was denominated.
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