Abstract:The pathogenic bacterium was evaluated according to the system analysis at the molecular level, which was acquired from the goat suspected of suffering from pneumonia in a field in Wushan County. The pathogenic bacterium was studied by means of morphological identification, drug sensitive test, and pathogenicity test after the separation and purification. Then the 16SrDNA was amplified, sequenced and analyzed using universal primers from the pathogenic bacteria. Result showed that the isolated strain was gram-negative and sensitive to many antibiotics, causing the respiratory disease of the goat. A sequence of 1 447 base pairs was obtained from the bacteria. Sequence compared to these derived from GenBank using BLASTn, and the closest sequence was the 16S rDNA gene of Moraxella osloensis strain, with a similarity of 99.9%, which was registered as JN084136. The pathogenic bacteria formed a separate with Moraxella according to the system development of the evolutionary tree that was based on the 16S rDNA genes sequences of the pathogenic bacteria and other related organisms, including the represent strain of Neisseria, Moraxella, Lampropedia, and Paracoccus. The investigation manifested that the pathogenic bacterium is Moraxella osloensis.
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