Abstract:In this study, we conducted an investigation of antimicrobial resistant characteristics to the clinical isolates of Salmonella enteritidis from chicken. Salmonella enteritidis were isolated from different sources in Shandong Province, and the susceptibility of isolates was analyzed by micro-dilution method to 19 antimicrobial agents. The resistant genes of strains were tested by PCR method, and the molecular types of strains from different sources were analyzed by pulse-field gel electrophoresis. The isolation rate of Salmonella enteritidis was 17.94% (178 strains). The resistance rate of strains to ampicillin, tetracycline, sulfamethoxazole, and nalidixic acid were 68.54%, 66.85%, 100%, and 100%, respectively. But the strains were still sensitive to gentamicin, chloramphenicol, and polymyxin, with a resistance rate below 5.62%. About 68.54% of strains (n=122) carrying the IntⅠ, blaTEM and tetA resistance genes. Isolates from different sources had four pulsed-field gel electrophoresis patterns. The isolates of Salmonella enteritidis were resistant to penicillins and tetracyclines in Shandong, and the main factor leading to antimicrobial resistance was the IntⅠ, blaTEM and tetA genes carrying in strains.
陆彦,吕安,赵红玉,侯晓林,吴国娟. 鸡源肠炎沙门氏菌对抗菌药物的耐药性分析[J]. 中国人兽共患病学报, 2014, 30(1): 17-22.
LU Yan,LU An,ZHAO Hong-yu,HOU Xiao-lin,WU Guo-juan. Analysis of antimicrobial resistance among Salmonella enteritidis from chicken. Chinese Journal of Zoonoses, 2014, 30(1): 17-22.
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