Association of PFGE with strains’ epidemic capacity in Vibrio cholerae O1 serogroup isolates
CHEN Ai-ping1,2,3,YANG Jin-song1,LI Qu-wen1,XU Hai-bin1, WANG Ling-lan1,ZHENG Jin-feng1,YAN Yan-sheng1
1.Fujian Center for Diseases Control and Prevention, Fuzhou 350001, China; 2.Priority Laboratory for Zoonosis Research of Fujian Province, Fuzhou 350001, China; 3.Teaching Base for School of Public Health, Fujian Medical University, Fuzhou 350001,China
Abstract:During the past five decades, the emerging Vibrio cholerae strains have caused three major waves of epidemic in Fujian Province, China. In this study, in order to investigate genetic relationship and genomic diversity of representative isolates from historic cholera outbreaks in Fujian, pulsed fielded gel electrophoresis (PFGE) was performed for V. cholerae O1 serogroup El Tor biotype strains, the association of PFGE genotypes with strains’ epidemic capacity was further analyzed in combination with epidemiological information. According to 100% similarity, a total of 61 PFGE patterns were identified in 77 strains of O1 serogroup, however, five major pulsotypes (G1-G5) were designated based on 90% similarity. Among the 52 strains of Ogawa serotype, there were 47 PFGE patterns that could be classified into 4 pulsotypes [G1(o)-G4(o)]. Meanwhile, fifteen PFGE patterns were observed in another 25 strains of Inaba serotype, mainly clustered in 2 pulsotypes [G1(i)-G2(i)]. Several predominant pulsotypes were determined in strains of diverse epidemic waves, however, sporadic isolates showed dispersed pulsotypes without clustering trends. Therefore, it is concluded from these data that genetic relationship was demonstrated among V. cholerae strains from three epidemic waves in Fujian, that novel predominant pulsotypes emerged in the process of epidemic when genetic variations occurred, and that there was geographic transmission association between cholera epidemics in prefectures. Particularly, this observation is also suggested that strains from pulsotype G2(o) of Ogawa serotype and pulsotype G1(i) of Inaba serotype are capable of causing persistent cholera, the latter is capable of inducing larger scale of disease burden.
陈爱平,杨劲松,李曲文,徐海滨,王灵岚,郑金凤,严延生. O1群霍乱弧菌PFGE分子分型和菌株流行能力关系研究[J]. 中国人兽共患病学报, 2014, 30(3): 259-262.
CHEN Ai-ping,YANG Jin-song,LI Qu-wen,XU Hai-bin, WANG Ling-lan,ZHENG Jin-feng,YAN Yan-sheng. Association of PFGE with strains’ epidemic capacity in Vibrio cholerae O1 serogroup isolates. Chinese Journal of Zoonoses, 2014, 30(3): 259-262.
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