Abstract：In this study, a TaqMan real-time PCR assay which targeted the highly repeated sequence of Schistosoma japonicum (S. japonicum) retrotransposon 2 was established to detect Schistosoma japonicum DNA in serum samples of host and to evaluate infectiosity. The method had high sensitivity and specificity, being able to detect 44.7 copies standard plasmid clones of DNA. The earliest time of detection of S. japonicum DNA was at the 3rd day post-infection, and the time points of early detection and detection threshold with different infectiosity were as follows: the target DNA in sera of rabbits infected with 30 cercariae (EPG=14) could not be detected until the 2nd week post-infection and the DNA level was 119.03 copies; the target DNA in sera of rabbits infected with 50 cercariae (EPG=24) and 100 cercariae (EPG=48) could be detected at the 1st week post-infection and the DNA level were 54.36 copies and 72.24 copies, respectively; the target DNA in sera of the rabbits infected with 200 cercariae (EPG=97) and 500 cercariae (EPG=232) could be detected at the 3rd day post-infection and the DNA level were 60.34 copies and 142.47 copies, respectively. Results showed that the serum DNA level of host infected with S. japonicum was positive correlation with infectiosity. The established TaqMan real-time PCR assay, as a sensitive, specific and convenient method, could quantificationally detect dynamic changes of serum DNA, and has potential application value in diagnosis of schistosomiasis and evaluation of infectiosity, providing a new assay for schistosomiasis diagnosis and evaluation of chemotherapy.
官威,许静,孙缓,梁松,董兰兰,夏超明. Real-time PCR法用于日本血吸虫感染宿主血清DNA的定量检测及其感染度的评价[J]. 中国人兽共患病学报, 2014, 30(3): 263-267.
GUAN Wei,XU Jing,LIANG Song,SUN Huan,DONG Lan-lan,XIA Chao-ming. Quantificational detection of Schistosoma japonicum DNA in serum of the host and assessment of infectiosity by real-time PCR. Chinese Journal of Zoonoses, 2014, 30(3): 263-267.
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2014, Vol. 30 
