Multiplex PCR for detection of Plasmodium falciparum and Plasmodium vivax
LI Huan1,SUN Fei2, WEN Lan3,WANG Xiao-chun1
1.Faculty of Laboratory Medicine, Xiangya School of Medicine, Central South University, Changsha 410013, China; 2.Hunan International Travel Health Care Center, Changsha 410016, China; 3. Changsha Center for Disease Control, Changsha 410001, China
Abstract:A multiplex PCR to detect Plasmodium falciparum (P. falciparum) and Plasmodium vivax (P. vivax) was established simply and rapidly in our study. Two pairs of primers (3 strips) were designed according to the sequences of the 18S rRNA of the two kinds of Plasmodium. The reacting system was optimized by analysis on the different primer concentration and annealing temperature. The patients’ blood samples were tested with the optimized multiplex PCR, and the limit of detection of this assay was estimated. The sensitivity and specificity were calculated using microscopy as the gold standard. The sizes of amplification products of multiplex PCR amplifying genomic DNA of P. falciparum and P. vivax were 431 bp and 341 bp, respectively. The limit of detection of this assay was 102 copies/reaction. The clinical sensitivity was 93.55% and the specificity was 70.83%. The positive predictive value (PPV) was 89.23% and the negative predictive value (NPV) was 80.95%. So we concluded that the multiplex PCR with high sensitivity could detect and identify malaria rapidly, which is suitable for primary Centers for Disease Prevention and Control.
李欢,孙菲,文岚,王晓春. 多重PCR快速检测恶性疟和间日疟的研究[J]. 中国人兽共患病学报, 2014, 30(3): 288-291.
LI Huan,SUN Fei, WEN Lan,WANG Xiao-chun. Multiplex PCR for detection of Plasmodium falciparum and Plasmodium vivax. Chinese Journal of Zoonoses, 2014, 30(3): 288-291.
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