Dynamic observation on levels of antibodies and cytokines in sera of BALB/c mice immunized with recombinant Bb(pGEX-Sj26GST-Sj32) vaccine of Schistosoma japonicm
XIANG Jin-ping1,LI Wen-gui2,ZHANG Li2
1.The People's Hospital of Dazu District, Chongqing 4000016, China; 2.Institute of Infectious and Parasitic Disease, the First Affiliated Hospital,Chongqing Medical University, Chongqing 400016, China
Abstract：To study the dynamic change of IgG, IgG subclasses,IgE,IgA,IL-10 and IL-12 in sera of BALB/c mice immunized with recombinant Bifidobacterium bifidum(Bb) pGEX-Sj26GST-Sj32 vaccine of Schistosoma japonicm, 96 BALB/c mice were immunized with the recombinant vaccine orally and intranasally, respectively, with 48 mice in each group.Then autopsies were made periodically to separate sera to determine the levels of IgG, IgG subclasses, IgE, IgA, IL-10 and IL-12 by ELISA at different time points of week 0,2,4,6,8,10,12,14,16,18,20 and 22 after immunization. It’s found that the levels of IgG, IgG1, IgG2a, IgG2b, IgG3, IgE, IgA, IL-10 and IL-12 in oral group reached to peaks on week 6, 8, 8, 4, 6, 12, 6, 12 and 6 respectively, while those in intranasal group reached the peaks on week 4, 2, 6, 2, 4, 8, 8, 6 and 6 respectively. It’s concluded that types Th1 and Th2 mixed type immune responses could be induced in mice by immunization with the recombinant Bb (pGEX-Sj26GST-Sj32) vaccine at early stage.
向进平,李文桂,张丽. 日本血吸虫重组Bb(pGEX-Sj26GST-Sj32)疫苗免疫BALB/c鼠血清抗体及细胞因子的动态观察[J]. 中国人兽共患病学报, 2014, 30(3): 300-305.
XIANG Jin-ping,LI Wen-gui,ZHANG Li. Dynamic observation on levels of antibodies and cytokines in sera of BALB/c mice immunized with recombinant Bb(pGEX-Sj26GST-Sj32) vaccine of Schistosoma japonicm. Chinese Journal of Zoonoses, 2014, 30(3): 300-305.
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2014, Vol. 30 
