Abstract:The aim of this study is to type the Yersinia pestis in Qinghai Plateau by DFR-based genotyping method and observe the spatial distribution characteristics. We typed 839 strains of Yersinia pestis from Qinghai according to DFR (different region) profiles, and used primers of 23 DFRs and PMT1 (plasmid validation primer) to amplify the strains’ DNA. Meanwhile, we analyzed the spatial distribution characteristics of the Yersinia pestis strains’ genomovars by geographic information system (GIS) of Qinghai Institute for Endemic Diseases Prevention and Control. Results showed that there were 11 genomovars--1b, 5, 7, 8, 10, 14, 21, 30, 32, 44, and 36, and the genomovar 32, 44, and 36 were new genomovars. Strains in Qinghai Plateau were given priority to genomovar 5 and 8. The most strains from the southern foothill of Qilian Mountain and the districts around Qinghai Lake belonged to genomovar 8, which covered 56.14% (471/839) of all the tested strains. The strains separated from the districts of Qingnan Plateau were mainly genomovar 5, accounting for 23.12% (194/839). All the strains separated from Microtus fuscus plague foci on Qingnan-Tibet Plateau were genomovar 14, accounting for 1.19% (10/839). So it is concluded that among Yersinia pestis strains from Qinghai, the strains of Marmot himalayan plague foci were given priority to genomovar 5 and 8, while the strains of Microtus fuscus plague foci were genomovar 14.
杨晓艳,魏柏青,辛有全,靳娟,何建,熊浩明,魏荣杰,祁美英,李存香,金泳,代瑞霞. 青海高原鼠疫菌DFR分型及空间分布特征的研究[J]. 中国人兽共患病学报, 2014, 30(4): 387-389.
YANG Xiao-yan,WEI Bai-qing,XIN You-quan,JIN Juan,HE Jian,XIONG Hao-ming, WEI Rong-jie,QI Mei-ying,LI Cun-xiang,JIN Yong,DAI Rui-xia. DFR genotyping analysis for Yersinia pestis in Qinghai Plateau and the observation on the spatial distribution characteristics. Chinese Journal of Zoonoses, 2014, 30(4): 387-389.
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