1.College of Food Science, Sichuan Agricultural University, Ya'an 625014, China; 2.Key Laboratory of Agricultural Products Processing and Preservation Engineering of Sichuan Province, Ya'an 625014, China
Abstract:Enrichment culture, selective plate separation, and biochemical identification methods were used for isolation of Campylobacter from 651 samples collected from chicken slaughter and processed for segments. And then three genes were selected for identification of the genus Campylobacter, which were the 16S rRNA (Campylobacter spp.), the Hip O (C. jejuni) and Ceu E (C. coli). Multiplex PCR assay (m-PCR) with three sets of primers was developed for identification of C. jejuni and C. coli. Finally, the isolated Campylobacter spp. were tested for the antimicrobial susceptibility against 8 kinds of antibiotics by using agar dilution method. The susceptibility results were determined by the standard of Clinical and Laboratory Standards Institute (CLSI, 2010). According to this method, 301 Campylobacter isolates were obtained from 651 samples, including 160 C. jejuni, 130 C. coli, and 11 unidentified Campylobacter isolates. The total separation rate of Campylobacter was 46.24%. The occurrences of Campylobacter contamination before slaughtering, after evisceration and chicken products were 56.10%, 31.00% and 17.00%, respectively. The results indicated that the resistant rates for Campylobacter isolates were 93.02% against ciprofloxacin. The Campylobacter isolates also exhibited a high rate of resistance to levofloxacin (87.71%), tetracycline (87.71%) and clindamycin (80.07%), showing different antimicrobial resistance to gentamicin (67.11%), erythromycin(49.50%), streptomycin(31.89%) and florfenicol (13.29%). It's indicated that Campylobacter spp. isolates from different processing steps were widespread and according to the antimicrobial susceptibility testing, and the resistant rates for Campylobacter spp. isolates were serious.
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