(1.Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 3500022, China; 2.Fujian International Travel Health Care Center, Fuzhou 350001, China)
Abstract:Hematophagy, blood-feeding, is a behaviour exhibited by most arthropod vectors of human pathogens. Hematophagous mosquitoes can transmit many diseases by blood-feeding. It causes a large number of deaths each year all over the world. The female generally feeds to repletion on a single blood meal and then proceeds to use this nutrition as the basis for the development of batch of eggs. A series of changes such as gene expression may occur after blood meal. Availability of several mosquitoes' genome sequences provides unique opportunities to study different aspects of biology, including identification of genes and pathways relevant to the developmental processes of individual life stages. However, development of novel vector control strategies requires a thorough detailed understanding of mosquito biology. Transcriptome analysis is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. It provides a comprehensive understanding of gene expression and its regulation. It also provides a far more precise measurement of levels of transcripts and their isoforms than any other methods. Transcriptome analysis has been successfully used in several mosquitoes and also achieved much useful information. Females of non-blood-feeding and blood feeding differ in many morphological, behavioural, and physiological traits, largely caused by differences in gene expression. The expression patterns of different genes are discussed in the context of female mosquitoes' physiological responses to blood feeding, including blood digestion, peritrophic matrix formation, egg development, and immunity. The changes of gene expression expose the main molecular differences between non-blood-feeding female and blood-feeding female. The present paper would summarize the current status of the transcriptome changes in mosquitoes induced by blood-feeding. It provides a valuable resource for the future studies.
肖菲菲, 黄恩炯, 张建明, 关雄. 蚊虫血餐前后转录组分析研究进展[J]. 中国人兽共患病学报, 2014, 30(5): 527-530.
XIAO Fei-fei, HUANG En-jiong, ZHANG Jian-ming, GUAN Xiong. Research advances on the analysis of blood-induced transcriptome changes in mosquitoes. Chinese Journal of Zoonoses, 2014, 30(5): 527-530.
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