Abstract:The differences of mip gene between the artificial and environmental water system were compared in our study. The 121 water samples of Legionella pneumophila (L. pneumophila) were collected from Guangzhou City and Xinhui City from the years of 2003 to 2008. All these isolates were divided into two groups according to their different environmental sources. Through the construction of phylogenetic tree of mip genes, gene polymorphic analysis, variance analysis, and neutrality test, the differences of mip gene between the two sources isolates have been compared. The distribution of mip genes, which were from different environmental L. pneumophila isolates in topology branch of the phylogenetic tree, was uneven. In the distribution of mip allele gene, there were 8 allele genotypes in the isolates from artificial water system, in which the allele 1 type was the dominant population with a count of 55.36%; there were 11 allele genotypes in the isolates from environmental water system, in which the allele 28 type was the dominant population with a count of 40%. In the analysis of gene polymorphic, compared with the average nucleotide polymorphism, the differences of isolates nucleotide polymorphism from artificial water system was greater than that from environmental water system. The differences from the two sources isolates were accounted for 14.43% in the total variance analysis of 121 L. pneumophila isolates. It has also been proved by the neutrality test that, in the 3′ terminal of mip gene obtained from artificial water system, the D value of Tajima test was significantly greater than zero (P<0.05); while in the same area, the difference of isolates obtained from environmental water system was not so significant. There were significant differences of L. pneumophila mip gene obtained between the artificial and environmental water systems. This might be due to the affection of artificial water system on the population size of L. pneumophila or on the balancing selected functions.
顾全, 胡朝晖, 严慧, 赵利伟, 范晓姗, 朱庆义. 人工水和自然水环境中嗜肺军团菌mip基因分型[J]. 中国人兽共患病学报, 2014, 30(5): 484-488.
GU Quan, HU Zhao-hui, YAN Hui, ZHAO Li-wei, FAN Xiao-shan, ZHU Qing-yi. Sequences-based analysis of mip gene of Legionella pneumophila isolated from artificial and natural water. Chinese Journal of Zoonoses, 2014, 30(5): 484-488.
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