Biological effects research of praziquantel derivatives anti-Schistosoma japonicum
SUN Huan1,DONG Lan-lan1,ZHAO Bo1,QIU Si-jie1,SUN De-qun2,XIA Chao-ming1
1. Department of Parasitology, School of Medicine, Soochow University, Suzhou 215123, China; 2. Marine College, Shandong University, Weihai 264209, China
Abstract：To observe the effects of 32 kinds of YCH-II-P series PZQ derivatives on juvenile and adult worms of Schistosoma japonicum (S. japonicum) in vitro, ICR mice were infected with S. japonicum cercariae. Mice were sacrificed on 6th week or 17 days after infection, and then the juvenile and adult schistosoma were collected by perfusion with ice-cold Hanks' balanced salt solution (HBSS, pH 7.2) from portal vein of liver. Worms were placed in DMEM medium containing different concentrations of drugs for l6 hours. The parasites were washed 3 times and soaked in drug-free medium for another 72 hours, observing under stereo-microscopy each day and by scanning electron microscope (SEM). Four kinds of YCH-II-P series effective compounds were P96, P125, P126, and P6 in vitro, and the parasites completely died in the concentration of 50 μmol/L. P96 and the critical lethal concentration against both adult and juvenile worms was 25 μmol/L. SEM showed the parasites cortex and gynecophoral ditch with seriously injures. These findings demonstrated that the P96 is effective PZQ derivatives on both juvenile and adult S. japonicum in vitro.
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2014, Vol. 30 
