1.Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China; 2.Microbiology Department, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hong Kong SAR 999077, China; 3.Division of Emergency Internal Medicine, Guiyang Medical College Affiliated Hospital, Guiyang 550004, China; 4.Division of Human Parasitology, Guiyang Medical College, Guiyang 550004, China
Abstract：In this study, a wild type Salmonella typhimurium (S. typhimurium) strain was isolated and identified in Hong Kong (S129), then the asdA gene was knocked out and replaced with kanamycin resistant gene in a Salmonella typhimurium strain S129 using the λ RED-mediated recombination method. The constructed mutant asdAΔS129 was validated by culturing in the presence or absence of 2,6-diaminopimelic acid (DAP) growth in vitro and evaluating its virulence in BALB/c mice challenge assay. Therefore, this study has demonstrated that an asdA mutant Salmonella typhimurium has been successfully constructed.
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2014, Vol. 30 
