Abstract:Effects of dihydroartemisinin (DHA) on the expression level of Alpha-7.3 giardin mRNA in C2 Giardia lamblia was investigated in this study to explore the damage to skeleton protein of C2 Giardia lamblia. Giardia lamblia was cultivated respectively for 2, 4, 8, and 12 hours with modified TYI-S-33 medium containing 100 μg/mL and 200 μg/mL DHA, while the control group performed in the same experimental conditions without DHA. The expressive quantity of Alpha-7.3 giardin mRNA was determined by using real-time reverse transcription PCR, and then we found that the expressive quantities of Alpha-7.3 giardin mRNA with DHA were significantly lower than those in the control group. It’s suggested that dihydroartemisinin has obvious inhibitory effect on the expression level of Alpha-7.3 giardin mRNA in C2 Giardia lamblia. The actions of dihydroartemisinin on skeleton protein of C2 Giardia lamblia are effective.
余源, 陈阳, 葛爽, 王洋, 李巍伟, 赵丽娜, 刘阿倩, 林志强, 高雪, 田喜凤. 双氢青蒿素对C2株蓝氏贾第鞭毛虫Alpha-7.3 giardin基因mRNA表达水平的影响[J]. 中国人兽共患病学报, 2014, 30(7): 669-672.
YU Yuan, CHEN Yang, GE Shuang, WANG Yang, LI Wei-wei, ZHAO Li-na, LIU A-qian, LIN Zhi-giang, GAO Xue, TIAN Xi-feng. Effects of dihydroartemisinin on the expression level of Alpha-7.3 giardin mRNA in C2 Giardia lamblia. Chinese Journal of Zoonoses, 2014, 30(7): 669-672.
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