Abstract:In this study, street trains were isolated from brain of suspected rabid dogs via mouse inoculation technique (MIT) and cell culture inoculation technique (CIT). Virus was identified by FAT, RT-PCR and sequencing. Then, the virus’ biological characteristics were analysed. The TCID50 test results demonstrated that viral titers were not high, which showed the BHK-21 cells was not the best host cell of rabies viral strain. The LD50 test results showed it was a strong strain of rabies virus. These results laid a strong foundation for rabies laboratory research in Fujian.
张建明, 邓艳琴, 王灵岚, 林代华, 杨秀惠, 严延生. 福建省狂犬病毒街毒株分离鉴定及生物学特性研究[J]. 中国人兽共患病学报, 2014, 30(7): 684-687.
ZHANG Jian-ming, DENG Yan-qin, WANG Ling-lan, LIN Dai-hua, YANG Xiu-hui, YAN Yan-sheng. Biological characterizations of street strain of rabies virus in Fujian Province,China. Chinese Journal of Zoonoses, 2014, 30(7): 684-687.
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