1.Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture /College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China; 2.Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China; 3.School of Basic Medical Science, Inner Mongolia Medical University, Hohhot 010110, China
Abstract：The purpose of this study was to clarify the strains of E. granulosus from sheep of Xiwuqi and people of Xilinhot in Inner Mongolia region and its genotypes. CO1 and ND1 of mitochondria gene were cloned and sequenced, and then they were analyzed by MegAlign of DNAStar5.0. Results showed that the length of CO1 and ND1 gene of E. granulosus, which were from sheep of Xiwuqi or people of Xilinhot, were 936 bp and 895 bp, respectively. The homology of CO1 gene sequences of E. granulosus strains from Xiwuqi and Xinjiang was 99.3%, while the homology of the corresponding gene sequences of E. granulosus from man of Xilinhot City and Xinjiang were 98.6%. ND1 gene of E. granulosus of sheep from Xiwuqi and human from Xilinhot were identical to ND1 gene of G1 type. All these indicated that the homology of E. granulosus from the two regions were high and the genotype were G1 type, which provided an important basis for the determination of strains, and it also had a great significance to prevent and control the disease.
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2014, Vol. 30 
