Isolation,identification and full-length genome sequence of EMCV from domesticated boar
CHANG Hong-tao1,LIU Hui-min2,ZHAO Jun1,CHEN Lu1,YANG Xia1, WANG Xin-wei1,YAO Hui-xia1,WANG Chuan-qing1
1.College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,China; 2.Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China
Abstract:Encephalomyocarditis virus (EMCV),named JZ1202,was isolated from domesticated boar in Henan,China. We performed the full-length genome sequencing and molecular characteristic analysis of the isolated strain. Results showed that the full-genome sequence of EMCV JZ1202 generated a sequence of 7 735 bp in length,and had 81.2%-99.9% nucleotide identity with other reference strains from different animals,but 99.4% with Chinese reference from pig. The phylogenetic tree was constructed based on the full-length genome; ORF and VP1 gene sequences identified EMCV was divided into G1,G2 and G3 groups; the strain JZ1202 belongs to G1 with other Chinese reference strains. Results identified that the EMCV infection could cause severe clinical symptoms in domesticated boar. Big regional differences exist in EMCV and the transmission is limited in a range of area. However,cross infection and prevalence of EMCV disease between domesticated boar and mice might exist. Mutation of some amino acid may occurred in EMCV infected domesticated boar.
[1]Zimmerman J,Allaire SD,Taylor DJ.Disease of swine[M]. 9th ed. Oxford: Blackwell Publishing Professional,2006: 331-336. [2]Liu HM,Yan Q,Zhao B,et al. Isolation,molecular characterization,and phylogenetic analysis of encephalomyocarditis virus from South China tigers in China[J]. Infect Genet Evol,2013,19: 240-243. DOI: 10.1016/j.meegid.2013.07.023 [3]Yuan WZ,Song QY,Zhang XY,et al. Isolation and molecular analysis of porcine encephalomyocarditis virus strain BD2 from northern China[J]. Infect Genet Evol,2014,21: 303-307. DOI: 10.1016/j.meegid.2013.11.014 [4]Deutz A,Fuchs K,Nowotny N,et al. Sero-epidemiological studies of zoonotic infections in hunters-comparative analysis with veterinarians,farmers,and abattoir workers[J]. Wien Klin Wochenschr,2003,115(3): 61-67. [5]Oberste MS,Gotuzzo E,Blair P,et al. Human febrile illness caused by encephalomycarditis virus infection. Peru[J]. Emerg Infect Dis,2009,15: 640-646. DOI: 10.3201/eid1504.081428 [6]Seaman JT,Boulton JG,Carrigan MJ. Encephalomyocarditis virus disease of pigs associated with a plague of rodents[J]. Aust Vet J,1986,63: 292-294. DOI: 10.1111/j.1751-0813.1986.tb08069.x [7]Billinis C. Encephalomyocarditis virus infection in wildlife species in Greece[J]. J Wild Dis,2009,45(2): 522-526. [8]Gelmetti D,Meroni A,Brocchi E,et a1. Pathogenesis of encephalomyocarditis experimental infection in young piglets: a potential animal model to study viral myocarditis[J]. Vet Res,2006,37: 15-23. DOI: 10.1051/vetres:2005041 [9]Bai J,Jiang FK,Li YF,et al. Analysis of the complete sequence of a porcine encephalomyocarditis virus isolate NJ08 from China[J]. Chin J Prev Vet Med,2011,33(5): 402-404. (in Chinese) 白娟,蒋康富,李玉峰,等.猪脑心肌炎病毒NJ08株基因组序列测定与分析[J].中国预防兽医学报,2011,33(5):402-404. [10]Ge XN,Yang HC,Guo X,et al. Isolation and characterization of porcine encephalomyocarditis virus[J]. Acta Veterinaria et Zootechnica Sinica,2007,38(1): 59-65. (in Chinese) 盖新娜,杨汉春,郭鑫,等.猪脑心肌炎病毒的分离与鉴定[J].畜牧兽医学报,2007,38(1):59-65. [11]Zhang GQ,Ge XN,Guo X,et al.Genomie analysis of two porcine encephalomyocarditis virus strains isolated in China[J]. Arch Virol,2007,152: 1209-1213. DOI: 10.1007/s00705-006-0930-9 [12]Zhang JL,Ge XN,Ma L,et al. Serological survey of EMCV infection in pigs on large-scale pig farms in China 2005-2006[J]. Chin J Vet Med,2007,43(1): 7-9. (in Chinese) 张家龙,盖新娜,马良,等.规模化猪场脑心肌炎病毒的血清学调查[J].中国兽医杂志,2007,43(1):7-9. [13]Zhao T,Zhang JL,Ge XN,et al. Comparative analysis virus isolates of the genomes of from porcine and encephalomyocarditis mouse origin[J]. Acta Veterinaria et Zootechnica Sinica,2009,40(6): 873-878. (in Chinese) 赵婷,张家龙,盖新娜,等.猪源和鼠源脑心肌炎病毒分离株基因组的比较分析[J].畜牧兽医学报,2009,40(6):873-878. [14]Chen JX,Shi KC,Huang SB,et al. Isolation of porcine encephalomyocarditis virus GXLC strain and analysis of the molecular characteristics of its 3D gene[J]. Chin Ani Husb Vet Med,2011,38(1): 81-87. (in Chinese) 陈进喜,施开创,黄胜斌,等.猪脑心肌炎病毒GXLC株的分离及其3D基因分子特征的分析[J].中国畜牧兽医,2011,38(1):81-87. [15]Knipe DM,Howley PM,Griffin DE,et al. Fields virology[M]. 4th ed. Philadelphia: Lippincott Williams & Wilkins Publishers,2001: 685-715. [16]Suh YS,Ha SJ,Lee CH,et al. Enhancement of VP1-specific immune response and protection against EMCV-K challenge by co-delivery of IL-12 DNA with VP1 DNA vaccine[J]. Vaccine,2001,19(15-16): 1891-1898. DOI: 10.1016/S0264-410X(00)00443-6 [17]Koenen F,Vanderhallen H,Castryck F,et al. Epidemiologic,pathogenic and molecular analysis of recent encephalomyocarditis outbreaks in Belgium[J]. Zentralbl Vet Med B,1999,46(4): 217-231. DOI: 10.1111/ j.0931-1793.1999.0_216.x [18]Lin WC,Liu YB,Cui SJ,et al. Isolation,molecular characterization,and phylogenetic analysis of porcine encephalomyocarditis virus strain HB10 in China[J]. Infect Genet Evol,2012,12: 1324-1327. DOI: 10.1016/j.meegid.2012.04.008