1.National Institute for Communicable Disease Control and Prevention / State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 2.Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China
Abstract：To investigate Legionella pollution prevalence and intracellular growth ability in pipe water from two airports in Shanghai, a total of 132 pipe water samples were collected from Shanghai port in 2010. The culture method was used to do qualitative and quantitative analysis of Legionella in pipe water samples. The serotypes of strains were determined by agglutinating. The intracellular growth ability of the Legionella isolates was tested by cell culture methods with mouse macrophage J774. Of the 132 water samples, 18.94% were observed to be positive with Legionella. The amount of Legionella in each positive sample was 50 to 1 320 CFU/200mL. Among the 25 isolates, 17 were Legionella pneumophila including 2 Lp1, one Lp5, one Lp7, and 13 Lp8, and 8 were Legionella micdadei. We selected five strains of different species and serotypes to infect J774, and the results showed that all the L. pneumophila strains have high intracellular growth ability, while L. micdadei has lower intracellular growth ability. It's suggested that there is high pollution prevalence of Legionella in pipe waters in Shanghai Port, which illuminated an urgent need for dealing with the potential risk of Legionella in pipe waters, through improved control and prevention strategies.
任红宇,田桢干,周海健,关红,邵祝军,秦天. 上海口岸管道水中军团菌污染状况及其细胞内生长能力研究[J]. 中国人兽共患病学报, 2014, 30(9): 929-933.
REN Hong-yu,TIAN Zhen-gan,ZHOU Hai-jian,GUAN Hong,SHAO Zhu-jun,QIN Tian. Contamination and intracellular growth ability of Legionella in pipe water system in Shanghai Port, China. Chinese Journal of Zoonoses, 2014, 30(9): 929-933.
[1]Fields BS, Benson RF, Besser RE. Legionella and Legionnaires' disease: 25 years of investigation[J]. Clin Microbiol Rev, 2002, 15(3): 506-526. DOI:10.1128/CMR.15.3.506-526.2002 [2]Diederen BMW. Legionella spp. and Legionnaires' disease[J]. J Infect, 2008, 56(1): 1-12. DOI: 10.1016/j.jinf.2007.09.010 [3]Declerck P, Behets J, Hoef VV, et al. Detection of Legionella spp. and some of their amoeba hosts in floating biofilms from anthropogenic and natural aquatic environments[J]. Water Res, 2007, 41(14): 3159-3167. DOI: 10.1016/j.watres.2007.04.011 [4]Blatt SP, Parkinson MD, Pace E, et al. Nosocomial Legionnaires' disease: aspiration as a primary mode of disease acquisition[J]. Am J Med, 1993, 95(1): 16-22. DOI: 10.1016/0002-9343(93)90227-G [5]Ito I, Naito J, Kadowaki S, et al. Hot spring bath and Legionella pneumonia: an association confirmed by genomic identification[J]. Intern Med, 2002, 41(10): 859-863. DOI: 10.2169/internalmedicine.41.859 [6]Lee HK, Shim JI, Kim HE, et al. Distribution of Legionella species from environmental water sources of public facilities and genetic diversity of L. pneumophila serogroup 1 in South Korea[J]. Appl Environ Microbiol, 2010, 76(19): 6547-6554. DOI: 10.1128/AEM.00422-10 [7]Kozak NA, Benson RF, Brown E, et al. Distribution of lag-1 alleles and sequence-based types among Legionella pneumophila serogroup 1 clinical and environmental isolates in the United States[J]. J Clin Microbiol, 2009, 47(8): 2525-2535. DOI: 10.1128/JCM.02410-08 [8]Liu HL, Lam LT, Hou CC, et al. A study of Legionella pneumophila in Tianjin, China[J]. Int J Environ Health Res, 2006, 16(1): 15-20. DOI: 10.1080/09603120500397565 [9]Lin H, Xu B, Chen Y, et al. Legionella pollution in cooling tower water of air-conditioning systems in Shanghai, China[J]. J Appl Microbiol, 2009, 106(2): 606-612. DOI: 10.1111/j.1365-2672.2008.04031.x [10]Shands KN, Ho JL, Meyer RD, et al. Potable water as a source of Legionnaires' disease[J]. JAMA, 1985, 253(10): 1412-1416. DOI: 10.1001/jama.1985.03350340064018 [11]Liu Z, Lin YE, Stout JE, et al. Effect of flow regimes on the presence of Legionella within the biofilm of a model plumbing system[J]. J Appl Microbiol, 2006, 101(2): 437-442. DOI: 10.1111/j.1365-2672.2006.02970.x [12]Wuerz TC, Mooney O, Keynan Y. Legionella pneumophila serotype 1 pneumonia in patient receiving adalimumab[J]. Emerg Infect Dis, 2012, 18(11): 1872-1874. DOI: 10.3201/eid1811.111505 [13]Ma XY, Wang YQ, Peng XM, et al. Investigation of an Legionnaires' disease outbreak associated with contaminated air conditioning system[J]. Chin J Epidemiol, 1998, 19(4): 200-205. DOI: 10.3760/j.issn:0254-6450.1998.04.002 (in Chinese) 马晓燕,王玉琴,彭晓旻,等.一起空调系统导致上呼吸道感染样军团菌爆发调查[J]. 中华流行病学杂志,1998,19(4):200-2-5. [14]Amemura-Maekawa J,Kikukawa K,Helbig JH, et al. Distribution of monoclonal antibody subgroups and sequence-based types among Legionella pneumophila serogroup 1 isolates derived from cooling tower water, bathwater, and soil in Japan[J]. Appl Environ Microbiol, 2012, 78(12): 4263-4270. DOI: 10.1128/AEM.06869-11 [15]Qin T,Xia J,Ren H,et al.Liver cirrhosis as a predisposing condition for Legionnaires' disease: a report of four laboratory-confirmed cases from China[J]. J Med Microbiol, 2012, 61: 1023-1028. DOI: 10.1099/jmm.0.040170-0 [16]Peng XM,Pei HS,Li XY,et al.Studies on Legionella-contamination to the air-conditioning cooling towers in big hotels and on its seroprevalence in the relatad populations in Beijing[J]. Chin J Epidemiol, 2000, 4: 289-291. DOI : 10.3760/j.issn:0254-6450.2000.04.014 (in Chinese) 彭晓旻,裴红生,黎新宇,等.北京市大饭店空调冷却塔军团菌污染现况及人群感染水平研究[J]. 中华流行病学杂志, 2000, 4: 289-291.
2014, Vol. 30 
