1.Chinese Center for Animal Disease Control & Prevention, Beijing 100125, China; 2.Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science /State Key Laboratory of Veterinary Etiological Biology, Lanzhou 730046, China
Abstract:Interactions between FMDV and cardiac cells are multifaceted and complex, these interactions leads to proteins alterations in cardiac cells inevitably. To understand the pathogenesis of myocarditis after FMDV infection in mice, the suckling mouse model for myocarditis induced by foot-and-mouth disease virus (FMDV) was established in this study. Suckling mice within 3 days old was selected to infect by FMDV. Myocarditis caused by FMDV in suckling mice was confirmed with clinical symptom monitor. The observation of Hematoxylin and eosin stain (H&E stain) and transmission electron microscopy (TEM) were performed after samples processing. According to conventional polymerase chain reaction (PCR) methods, primers of VP1 gene was designed, synthesised and specific FMDV VP1 gene was amplified from the heart muscle of suckling mice. The results indicated that suckling mice appeared low spirit condition, dyspnea, and dull reaction within 36 hours after challenge with FMDV. Infiltration of inflammatory cells and dissolution of myocardial fibers were observed with H&E stain and TEM. Special target gene of FMDV was amplified from the heart of infected group. Obvious inflammation in the heart of suckling mice caused by FMDV was observed. It's suggested that suckling mouse model for myocarditis induced by FMDV was established successfully, which would lay the foundation for researches of myocarditis mechanism in young cloven-hoofed animals.
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