Abstract：To observe the dynamic changes of splenocyte proliferation, subsets and apoptosis in mice immunized with recombinant Bifidobacterium bifidum (pGEX-Sj26GST) of Schistosoma japonicum, the mice were subcutaneously (SC group) and intranasally (IN group) immunized, respectively. Four mice from each group were sacrificed in every 2 wk during 0-20 wk after immunization. Splenocyte proliferation was investigated by MTT colorimetric assay, subsets of CD+4 and CD+8 T cells and apoptosis of splenocytes by FACsort flow cytometry. In SC group, unstimulated and stimulated with SjAWA, the level of splenocyte proliferation significantly increased at 4-20 wk after vaccination and increased markedly at 4-18 wk stimulated with ConA, both of which peaked at 8 wk; in IN group, the proliferation level of splenocyte cultured with SjAWA and ConA significantly increased during the 4-18 wk, 2-10 wk and 14-18 wk, 2-8 wk and 12-18 wk, respectively, and all reached the maximum at the 4 wk after immunization (P<0.01 or P<0.05). CD+4 subsets increased obviously during 2-14 wk, 2 wk and 6-16 wk respectively, and reached the peak at 8 wk (P<0.01 or P<0.05) in both group, while CD8+ subsets rose lightly during 2-20 wk in both group, and reached the maximum at 8 wk (SC group) and 6 wk respectively (P>0.05). Whether unstimulated or stimulated with ConA, the level of splenocyte apoptosis of which remarkably increased at 2-4 wk and 2-6 wk separately in SC group, and both peaked at 2 wk (P<0.01 or P<0.05 ); in IN group, the level of splenocyte apoptosis all increased at 4 wk and reached the maximum at the same time. In summary, by inducing the proliferation of splenocytes, increasing CD4 + T cells and decreasing splenocyte apoptosis, the rBb (pGEX-Sj26GST) vaccine plays a critical role in the protective immune response.
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2014, Vol. 30 
