Abstract：To explore the influence of Trichinella spiralis ES antigen on the expression of mRNA of TLR4, NF-κB and AP-1 in DC 2.4, we set up the following groups: blank control group, LPS group, ES antigen group, ES antigen+LPS/ES antigen group, and LPS+LPS/ES antigen group, which were presented by expression level of TLR4, NF-κB and AP-1 gene mRNA, and detected by fluorescence quantitative PCR (Real-time FQ-PCR) technology. Results showed that expression level of TLR4, NF-κB and AP-1 mRNA in pre-treatment of ES antigen and LPS group were significantly lower than that of ES and LPS group (P<0.05), respectively. We confirmed that the expression of mRNA of TLR4, NF-κB and AP-1 of DC2.4 could be suppressed by constant stimulation or combined effect of ES antigen and LPS.
刘畅, 韩彩霞, 李巍, 李晓云, 路义鑫, 王泽, 唐颖, 宋铭忻. 旋毛虫ES抗原对DC2.4细胞TLR4、NF-κB和AP-1 mRNA表达的影响[J]. 中国人兽共患病学报, 2014, 30(11): 1116-1120.
LIU Chang, HAN Cai-xia, LI Wei, LI Xiao-yun, LU Yi-xin, WANG Ze, TANG Ying, SONG Ming-xin. Effect of ES antigen of Trichinella spiralis on expression of mRNA of TLR4, NF-κB and AP-1 in DC 2.4. Chinese Journal of Zoonoses, 2014, 30(11): 1116-1120.
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2014, Vol. 30 
