Correlation between Pneumosystis colonization/infection and respiratory diseases
ZHANG Nan1, 2, TAN Li-si3, QIN Zheng4, MA Su-li1, GUO Jiu-ying1, FAN Hua1, AN Chun-li1
1.Department of Pathogen Biology, School of Basic Medical Science, China Medical University,Shenyang 110001, China;
2.Centers for Disease Control and Prevention, Shanghai 201800, China;
3.Department of Periodontics, School of Stomatology, China Medical University, Shenyang 110001, China;
4.Institute for Respiratory Diseases, China Medical University, Shenyang 110001, China
In the study, we investigated the correlation between respiratory diseases and Pneumocystis colonization or infection. Species-specific primers targeted the 16S rRNA gene were designed, the loop mediated isothermal amplification (LAMP) for testing Pneumosystis was established and its sensitivity and specificity was identified. The LAMP was performed to detect Pneumocystis gene in induced sputum from patients suffer from respiratory diseases. The correlation of the Pneumosystis colonization and respiratory diseases was analyzed. Results showed that the minimum amount of LAMP amplified gene was 50 copies/mL; there was no cross-reaction in other 8 kinds of respiratory pathogens. A total of 98 sputum specimens were detected and the positive rate of Pneumosystis gene was 63.3%. The positive rate of Pneumocystis gene was 53.2% in COPD patients and 71.2% in non-COPD patients. The positive rate was 66.8% during COPD acute phase and 46.9% during stability course; the difference is no statistically significant. Merger respiratory tract infection was detected in 63 cases, and the positive rate was 60.3% in co-infected patients and 75.0% in non-co-infected patients, showing no statisticaly difference. Positive rate of Pneumocystis gene was 72.5% in the patients with changed lung imaging and 53.2% in those with no lung imaging change, both having significant differences. The detection rate was 80.0% when the CD4 + T lymphocyte count was lower, and 60.3% in the patients whose CD4+ T lymphocyte count was normal. Pneumocystis genetic testing results showed that both had significantly statistical significance (P>0.05) in the patients with increased 1,3-beta-D-glucan content or normal. In conclusions, the detection rate of Pneumocystis gene is higher in patients with respiratory diseases. Its mechanism remains to be further research. Pneumocystis infection or colonization is influenced by the amount of CD4+ T cells.
张楠, 谭丽思, 秦铮, 马素丽, 国九英, 樊华, 安春丽. 肺孢子菌感染或定植与呼吸系统疾病的相关性探讨[J]. 中国人兽共患病学报, 2014, 30(11): 1090-1096.
ZHANG Nan, TAN Li-si, QIN Zheng, MA Su-li, GUO Jiu-ying, FAN Hua, AN Chun-li. Correlation between Pneumosystis colonization/infection and respiratory diseases. Chinese Journal of Zoonoses, 2014, 30(11): 1090-1096.
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