Molecular biological identification on skin infection of Mycobacterium chelonae
ZHANG Zhong-kui1, KANG Dao-xian2, LIU Tai-hua1, ZHOU Zhou1, WANG Chun-mei1, WANG Jun1, TANG Li-jun1, CHENG Ye-hong1, RAN Yu-ping2
1.Department of Dermatology, Chengdu Military General Hospital, Chengdu 610083, China; 2.Department of Dermatovenerology, West China Hospital, Sichuan University, Chengdu 610041, China
Abstract：Molecular biological study was applied in identifying clinical isolated strain of Mycobacterium chelonae. The wound surface secretion was taken from the focus of suppurative infection in the right leg of a senile female patient with diabetes and was cultured in sabouraud’s agar medium at the temperature of 28℃ for 4 days; the culture was performed for twice, smooth, wet and white nonphotochromogens colonies were gained, which were positive in Gram stain and acid-fast stain while negative in fungus microscope examination and culture. Histopathologic study of the skin lesion showed a chronic suppurative infection of subcutaneous tissue, inflammatory cell infiltration and local small abscess formation, while positive in acid-fast stain and negative in PAS stain. Molecular biological identification of 16S-rRNA, hsp65 and rpoB was performed and Blast intercomparasion was made in GenBank. The sequencing result of 16S-rRNA was 100% in homology with Mycobacterium chelonae AB548610.1 while the sequencing results of rpoB and hsp65 were 99% in homology with Mycobacterium chelonae AB548610.1. The strain is conformed to be Mycobacterium chelonae.
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2014, Vol. 30 
