1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention / Key Laboratory of Parasite and Vector Biology, MOH / WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China; 2. Department of Pathogenic Biology, the Second Military Medical University, Shanghai 200433, China
Abstract：To explore the feasibility of DNA barcoding in the identification of sandflies, the mitochondrial cytochrome c oxidase subunit I (COI) genes of 9 dominant species in China from three genera in the Phlebotominae were studied. The intraspecies and interspecies genetic distances were calculated using the Kimura-2-parameter model, and the phylogenetic trees were reconstructed by neighbor-joining (NJ) method. Results showed that all of sandflies species were successfully distinguished by the phylogenetic trees. The average intraspecific genetic distance (0.8%) was much less than the average interspecific genetic distance (11.2%). The study based on COI sequences indicates that the sandflies from China could be well distinguished with the commonly used COI barcodes, which are potentially well utilized in sandflies species identification.
周正斌,张仪,吕山,施文琦,金长发,朱淮民. DNA条形码技术鉴定我国部分白蛉蛉种[J]. 中国人兽共患病学报, 2014, 30(12): 1209-1213.
ZHOU Zheng-bin,ZHANG Yi,LV Shan,SHI Wen-qi,JIN Chang-fa,ZHU Huai-min. Species identification of partial sandflies from China with DNA barcoding. Chinese Journal of Zoonoses, 2014, 30(12): 1209-1213.
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2014, Vol. 30 
