1.Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China; 2.Xiacheng District Center for Disease Control and Prevention, Hangzhou 310003, China
Abstract：We investigated the prevalence of New Delhi metallo-β-lactamase-1 (NDM-1) producing strains by screening the blaNDM-1-positive gene using TaqMan-MGB real-time PCR in clinical isolates collected from different areas of Zhejiang Province in 2013. Sequencing of the blaNDM-1 gene, multilocus sequence typing (MLST) and 30 drug resistant gene tests were performed for the blaNDM-1-positive strains. Antimicrobial susceptibility of the blaNDM-1-positive strains was analyzed by the VITEK 2 Compact System. The modified Hodge test and imipenem (IPM)-EDTA double-disk synergy test were performed to detect the carbapenemase and metallo-β-lactamase. One NDM-1-producing Klebsiella pneumonia strain was identified in 1 450 isolates, and this Klebsiella pneumonia strain was isolated from stool sample of an eleven-month-old baby who was suspected of viral diarrhea. The homology with NDM-1 gene from GenBank was up to 100%. The blaNDM-1-positive K. pneumonia strain was typed as ST1416, and produced the carbapenemase and metallo-β-lactamase. It was susceptible to levofloxacin and trimethoprim/sulfamethoxazole, intermediate susceptibility to ciprofloxacin, but resistant to 18 other antibiotics. Additional drug resistant genes including blaSHV, blaCTX, blaDHA, blaSPM, blaCMY-2, blaIMP, blaGES, blaGIM, blaTEM, blaAmpC, blaOXA-48, aadA1, aacA4 and aphA1 were detected in this positive isolate, and the phenotype was coincide with its genotype. It is the first time to identify the New Delhi metallo-β-lactamase-1 (NDM-1) producing K. pneumonia from Enterobacteriaceae in Zhejiang Province. The faster transfer of NDM-1 gene between the Enterobacteriaceae implies that the continuous surveillance for monitoring NDM-1-producing bacteria is necessary.
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2015, Vol. 31 
