Abstract:In order to etiologically diagnose and analyse the suspected patients of brucellosis and provide etiologic basis for the confirmation of patients and the control of human brucellosis in Guizhou Province, conventional and molecular techniques were used to identify suspicious bacteria strains isolated from the suspected patients of brucellosis. Results showed that a total of 13 Brucella suspicious bacteria strains were isolated and 11 Brucella suspicious strains were identified as B. melitensis biotype 3 by conventional tests, with 2 strains unable to be identified. Genus specific BCSP31-PCR identified all the 13 bacteria strains as Brucella spp. Species-specific AMOS-PCR further identified the 11 strains as B. melitensis, and the 2 strains unidentified with conventional tests were also unable to be identified with AMOS-PCR. Results of this study suggested that B. melitensis biotype 3 were the main epidemic Brucella in Guizhou Province in 2013, accounting for 84.6% (11/13), which provided etiological basis for the diagnosis of patients and the control of human brucellosis.
刘英,王月,马青,李世军,黄艳,周敬祝,余春,田克诚,唐光鹏,王定明. 贵州省2013年人间布鲁氏菌病病原体种/型鉴定与分析[J]. 中国人兽共患病学报, 2015, 31(1): 45-48.
LIU Ying,WANG Yue,MA Qing,LI Shi-jun,HUANG Yan,ZHOU Jing-zhu, YU Chun,TIAN Ke-cheng,TANG Guang-peng,WANG Ding-ming. Etiologic identification and analysis of the human brucellosis in Guizhou Province, China, 2013. Chinese Journal of Zoonoses, 2015, 31(1): 45-48.
[1]Shang DQ. Brucellosis raging again and the reasons[J]. Chin J Contr Endem Dis, 2001, 16(1):29-34. (in Chinese)尚德秋. 布鲁氏菌病再度肆虐及其原因[J]. 中国地方病防治杂志,2001, 16(1):29-34. [2]Li SJ, Wang Y, Chen H, et al. Isolation and Identification of Brucella melitensis firstly isolated from goat in Guizhou province[J]. Chin J Zoonoses, 2011, 27(6):515-518. (in Chinese) 李世军,王月,陈红,等. 贵州省首次从山羊分离到布鲁氏菌及其种型鉴定[J]. 中国人兽共患病学报, 2011, 27(6):515-518. [3]Li SJ, Wang Y, Chen H, et al. Etiologic diagnosis and analysis of the first case of human brucellosis in Guizhou province[J]. Chin J Endem Dis, 2012, 31(5):69-71. (in Chinese) 李世军,王月,王定明,等. 贵州省首例人间布鲁氏菌病病例的病原学诊断与分析[J]. 中国地方病学杂志, 2012, 31 (5) : 69-71. [4]Disease Prevention and Control Bureau, the Ministry of Health. Brucellosis prevention manual[M]. Beijing: People’s Publishing House. 2008: 17-29. (in Chinese) 卫生部疾病预防控制局.布鲁氏菌病防治手册[M]. 北京:人民出版社, 2008:17-29. [5]Li LY, Qiu HY, Shang DQ. A study on the PCR about primers of 31 kDa protein gene for B. abortus[J]. Chin J Endem Dis, 2000, 15(4):196-198. (in Chinese) 李兰玉,邱海燕,尚德秋.牛种布鲁氏菌31 kDa蛋白基因引物的PCR试验(I) [J]. 中国地方病防治杂志, 2000, 15(4):196-198. [6]Hai J, Cui BY, Zhao HY. Study on identification of Brucella spp. using AMOS PCR[J]. Chin J Zoonoses, 2009, 25(2):107-109. (in Chinese) 姜海,崔步云,赵鸿雁. AMOS-PCR对布鲁氏菌种型鉴定的应用[J]. 中国人兽共患病学报, 2009, 25(2):107-109. [7]Cui BY. Brucella epidemic situation and vaccine research in China[J]. Chin J Epidemiol, 2012, 31(4):355-356. (in Chinese) 崔步云. 关注中国布鲁杆菌病疫情发展和疫苗研究[J].中国地方病学杂志, 2012, 31(4):355-356. [8]Cui BY, Yin JM, Li LY, et al. Typing of Brucella Isolates by repetitive element sequence-based polymerase chain reaction[J]. Dis Surveill, 2005, 20(8):397-400. (in Chinese) 崔步云,尹继明,李兰玉,等. 布鲁氏菌的Rep-PCR分型研究[J]. 疾病监测, 2005, 20(8):397-400. [9]Chen JD, Deng XL, Ke BX, et al. Etiological analysis of brucellosis in Guangdong province[J]. Dis Surveill, 2008, 23(5):227-229. (in Chinese) 陈经雕,邓小玲,柯碧霞,等. 广东省布鲁氏菌病病原学特征分析[J]. 疾病监测, 2008, 23(5):227-229. [10]Wang L, Ma GZ. The research for diagnosing brucellosis patients with PCR technology[J]. Chin J Endem Dis, 2004, 19(2):65-67. (in Chinese) 王丽,马国柱. PCR技术用于布鲁氏菌病的诊断研究[J].中国地方病杂志, 2004, 19(2):65-67 . [11]Peng XB, Chen JS, Xia YC, et al. A Multi-PCR assay for differentiating the strains of B. abortus, B. melitensis and B. suis[J]. Chin J Vet Drug, 2010, 44(2):12-14. (in Chinese) 彭小兵,程君生,夏业才,等. 多重PCR方法鉴别牛、羊、猪种布鲁氏菌株[J]. 中国兽药杂志, 2010, 44(2):12-14. [12]Di Giannatale E, De Massis F, Ancora M, et al. Typing of Brucella field strains isolated from livestock populations in Italy between 2001 and 2006[J]. Vet Ital, 2008, 44(2):383-388. [13]Matope G, Bhebhe E, Muma JB, et al. Characterization of some Brucella species from Zimbabwe by biochemical profiling and AMOS-PCR[J]. BMC Res Notes, 2009, 22(2):261. DOI:10.1186/1756-0500-2-261