Abstract：To investigate the serotypes and virulence genes of extraintestinal pathogenic Escherichia coli (ExPEC) in Qinhuangdao City, China, a total of 30 tissue samples were collected from pigs during 2013 to 2014 and 8 isolates of ExPEC were isolated which were identified by colony morphology, culture characteristics identification, and biochemical observation. The O serogroups were identified by agglutination with specific antisera. All strains were screened for virulence genes by PCR. Three O serogroups were identified for 5 E. coli isolates, of which O53, O93 and O157 were dominant serogroups. In addition, the virulence genes were detected by PCR and the results showed that ler, iutA, irp2, fyuA, and astA were positive in the 8 isolates by 100%, 75.0%, 50.0%, 50.0%, and 25.0%, respectively. It is concluded that O53, O93 and O157 are dominant serogroups associate with the disease of swine. Isolates carrying pathogenicity islands gene irp2, fyuA and ler were proved to be pathogenic to mice.
马增军,芮萍,逯春香,杨彩然,刘谢荣,王秋悦,刘曜综,张建文. 猪源肠外致病性大肠杆菌的分离与鉴定[J]. 中国人兽共患病学报, 2015, 31(2): 130-134.
MA Zeng-jun,RUI Ping,LU Chun-xiang,YANG Cai-ran, LIU Xie-rong,WANG Qiu-yue,LIU Yao-zong,ZHANG Jian-wen. Isolation and identification of extraintestinal pathogenic Escherichia coli from swine. Chinese Journal of Zoonoses, 2015, 31(2): 130-134.
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2015, Vol. 31 
