Isolation and identification of the first strain of Enterohemorrhagic Escherichia coli O157∶H7 isolated from a patient with infectious diarrhea in Guizhou Province, China
Abstract:In this study, we aimed to isolate and identify Enterohemorrhagic Escherichia coli O157∶H7 from a patient with infectious diarrhea in Guizhou Province, and then detect virulence genes. Feces from a diarrhea patient were enriched in mEC broth. The E. coli O157 was primary screened by Gold-immunochromatograpy assay (GICA), then followed by immunomagnetic bead separation (IMS) for the isolation of EHEC O157 from positive enrichment medium. Isolates were conducted with biochemical identification, sero-groups testing, specific PCR identification and virulence genes detection. Results showed that GICA was positive and EHEC O157 suspicious colonies were cultured by IMS. The strain was identified as E. coli O157∶H7 by sero-type and specific PCR. The virulence genes of eaeA, stx2 and hly were tested positive, and the stx1 was negative. The laboratory diagnostic result showed that the isolate from a patient with infectious diarrhea was EHEC O157∶H7. It is the first EHEC O157∶H7 strain isolated from a patient with infectious diarrhea in Guizhou Province.
韦小瑜,游旅,田克诚,唐光鹏,王定明. 贵州省首次从腹泻病例中检出肠出血性大肠杆菌O157∶H7[J]. 中国人兽共患病学报, 2015, 31(2): 183-185.
WEI Xiao-yu,YOU Lü,TIAN Ke-cheng,TANG Guang-peng,WANG Ding-ming. Isolation and identification of the first strain of Enterohemorrhagic Escherichia coli O157∶H7 isolated from a patient with infectious diarrhea in Guizhou Province, China. Chinese Journal of Zoonoses, 2015, 31(2): 183-185.
[1]Han R, Fang HL, Guo F, et al. Initial adherence of EHEC[J]. Progr Mod Biomed, 2014, (2): 372-376. (in Chinese) 韩燃, 房华丽, 郭峰,等.EHEC的新型粘附因子研究进展[J].现代生物医学进展, 2014, (2):372-376. [2]Chen DL, Xu YM, Luo X, et al. Detection rfbO157, fliCH7, hlyA, eaeA, stx2 and mutation of stx2 genes of EHEC O157∶H7 by PCR and meaning[J]. Chin J Health Lab Technol, 2005, 15(10): 1197-1201. (in Chinese) 陈道利, 许彦梅, 罗霞,等.PCR方法检测EHEC O157∶H7的rfbO 157、fliCH7 、hlyA、eaeA、stx2及其变种基因[J].中国卫生检验杂志, 2005, 15(10):1197-1201. [3]Wang L, Ye CY, Zao AL, et al. Identification of a non-Shiga toxin-producing Enterohemorrhagic Escherichia coli O157∶H7 strain[J]. Dis Surveillance, 2007, 22(7): 441-443. (in Chinese) 王蕾, 叶长芸, 赵爱兰,等.一株不产志贺毒素的肠出血性大肠杆菌: 菌株的鉴定[J].疾病监测, 2007, 22(7):441-443. [4]Tyler SD, Johnson WM, Lior H, et al. Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment length polymorphism analysis[J]. J Clin Microbiol, 1991, 29(7): 1339-1343. [5]Wei XY, You L, Tian KC, et al. Epidemic feature and bacteria etiological distribution for infectious diarrhea cases in Guiyang city[J]. J Appl Prev Med, 2012, 18(3): 133-136. (in Chinese) 韦小瑜, 游旅, 田克诚.贵阳市例夏秋季腹泻病例流行特征及细菌病原分布[J].应用预防医学, 2012, 18(3):133-136. [6]Yan Y, Guo J, Tian KC, et al. Analysis on the pathogeny of viral diarrhea in summer and fall of 2010 in Guiyang districts[J]. Mod Prev Med, 2012, 18(3): 133-136. (in Chinese)阎岩,郭军,田克诚,等.贵阳地区年夏秋季病毒性腹泻的病原谱分析[J].现代预防医学, 2013, 40(7):1201-1203. [7]Diao LQ, Wang W, Xu JG, et al. Gold-immunochromatography assay for Escherichia coli O157[J]. Dis Surveillance, 2007, 22(7):438-440. (in Chinese) 刁琳琪,王伟,徐建国,等.胶体金标记免疫层析法检测大肠杆菌[J].疾病监测, 2007, 22(7):438-440. [8]Xu YM, Xu JG. Application of PCR in the diagnosis of Entero-hemorrhagic E. coli O157∶H7[J]. Chin J Zoonoses, 2003, 19(6): 88-92. (in Chinese) 许彦梅,徐建国.PCR方法在出血性大肠杆菌:诊断中的应用[J].中国人兽共患病杂志, 2003, 19(6):88-92. [9]Wang T, Liu LY, Wang P, et al. Virulent genes of Escherichia coli O157∶H7 strains isolated from China[J]. Chin J Zoonoses, 2010, 26(6): 515-518. (in Chinese) 王涛,刘丽云,王娉,等.我国分离的大肠埃希菌:的毒力基因检测分析[J].中国人兽共患病学报, 2010, 26(6):515-518. [10]Tan JH, An TZ, Yue ZP, et al. Etiological investigation of colibacillosis in piglets in Guizhou province[J]. Chin J Vet Sci Technol, 2001, 31(1):15-17. (in Chinese) 谭建华, 安铁洙, 岳占碰,等.贵州省仔猪大肠杆菌病病原学的调查研究[J].中国兽医科技, 2001, 31(1):15-17.