Abstract：In order to investigate gene sequences and evolution characteristics of rabies virus and provide theoretical information, the complete genome of the first rabies virus isolate from Fujian was sequenced and analyzed. In this study, we extracted viral RNA from cell culture directly, and then the segment RT-PCR were used for gene sequence amplification; thirdly, the obtained gene fragments were molecularly cloned and sequenced; at last, these fragments consisted of whole genome sequences were analyzed. The entire genome nucleotide sequence was obtained with no insertion and deletion in the coding region after the application of designed rabies primers. Mutations in the N protein antigenic sites occurred mainly in B-cell epitope a 379 V → L; the amino acid for glycoprotein antigenic sites I (231-bit) was L; antigenic site II 199 at sites R → G mutation occurred; antigenic site III 333 bit arginine (R). These results laid a foundation for further research on the mutation, dissemination, and mechanisms of rabies virus.
张建明, 王宇平, 邓艳琴, 王灵岚, 严延生. 福建省首例狂犬病街毒分离株基因组序列测定及分析[J]. 中国人兽共患病学报, 2015, 31(4): 311-314.
ZHANG Jian-ming, WANG Yu-ping, DENG Yan-qin, WANG Ling-lan, YAN Yan-sheng. Sequencing and analysis of the complete genome of the first rabies virus isolate from Fujian, China. Chinese Journal of Zoonoses, 2015, 31(4): 311-314.
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2015, Vol. 31 
