Abstract:We investigated Borrelia burgdorferi infection in ticks and rodents captured in Maijishan region and identified their genotypes in this study. Ticks were collected by dragging a cloth over the vegetation, rodents were collected by snaps. After species identification, DNA of pathogen was isolated from samples and detected by nested PCR targeting 5S-23S rRNA intergenic spacer of Borrelia burgdorferi. Positive samples were further analysed by restriction fragment length polymorphism (RFLP). Results showed that a total of 1 273 ticks including 1 126 nymphal ticks and 147 adult ticks in 4 species; 42 rodents including 4 species were tested for evidence of Borrelia burgdorferi by nested PCR; 15 adult tick samples, 9 rodents samples and 13 groups of nymphal ticks showed positive. The positive rates were different among the different rodent species, with Apodemus agrarius having the highest positive rates. RFLP analysis indicated that Borrelia burgdorferi in samples belonged to the genotype either B. garinii or B. afzelii. It's suggested that Borrelia burgdorferi exists in Maijishan region and there were at least two genotypes.
张继军, 张芳, 李璐, 刘增加. 甘肃麦积山区媒介及宿主动物中莱姆病螺旋体检测及基因型别研究[J]. 中国人兽共患病学报, 2015, 31(4): 357-360.
ZHANG Ji-jun, ZHANG Fang, LI Lu, LIU Zeng-jia. Borrelia burgdorferi infection and their genotypes in ticks and rodents collected from Maijishan region, Gansu Province, China. Chinese Journal of Zoonoses, 2015, 31(4): 357-360.
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