DNA barcoding and its applications in parasitology
XU Wu-jie1, WANG Yan2, 3, ZOU Jie-xin1, 3, ZHU Chun-chao1, 3, ZHOU Xian-min1, 3
1.Department of Parasitology, Medical College, Nanchang University, Nanchang 330006, China; 2.Medical Genetics and Cell Biology Laboratory, Medical College, Nanchang University, Nanchang 330006, China; 3.Key Laboratory of Poyang Lake Environment and Resource Utilization Supported by the Ministryof Education, Nanchang University, Nanchang 330047, China
Abstract：Parasite is a specific organism group closely related to human health, and its taxonomy and classification are important foundation for parasitological research. "DNA barcoding" is a rapid and accurate method for species identification through a standard DNA fragment. It is a new developed molecular marker technology after RFLP, PCR-RFLP, SSR-PCR, ISSR etc., based on the powerful computers and widely using internet. As it overcomes the negative influence of phenotypic plasticity and genetic variability, and is not restricted by gender and development stage, it has become the current hot spot in taxonomical study. In this paper, we reviewed the evolution of DNA barcode concept and technology, basic methodology, and current controversy, and described it's application in identification of protozoa, helminth and Arthropoda, the development of DNA barcode technology, and its application in parasite research.
徐武杰, 汪雁, 邹节新, 朱春潮, 周宪民. DNA条形码及其在寄生虫学中的应用[J]. 中国人兽共患病学报, 2015, 31(4): 365-370.
XU Wu-jie, WANG Yan, ZOU Jie-xin, ZHU Chun-chao, ZHOU Xian-min. DNA barcoding and its applications in parasitology. Chinese Journal of Zoonoses, 2015, 31(4): 365-370.
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