Abstract：In this study, we identified bacteria strain from the blood sample of a patient in a suspected epidemic of human Streptococcus suis infections, investigated the etiologic characteristics and provided etiologic basis for epidemic determination and the diagnosis and treatment of the patient. Suspicious bacteria strain of S. suis from the blood sample of a patient in a suspected epidemic of Streptococcus suis infections was identified using gram staining, serum agglutination test and biochemical test. Streptococcus suis species specific gene 16S rRNA, Streptococcus suis serotype 2 specific virulence gene capsule polysaccharide 2J (cps2J) and virulence gene muramidase-released protein (mrp), hemolysin gene (sly) and extracellular factor protein gene (ef) were further detected by PCR. The suspicious bacteria strain of S. suis was identified as Streptococcus suis type 2 by conventional methods and PCR. PCR detection results showed that 16S rRNA, cps2J, mrp, sly and ef genes of S. suis were positive. This epidemic was caused by human infection with virulent S. suis type 2, which provided etiologic basis for the epidemic determination and the diagnosis and treatment of the patient.
马青, 李世军, 刘英, 田克诚, 唐光鹏, 王定明. 贵州省一例人感染猪链球菌病例的病原学检测与分析[J]. 中国人兽共患病学报, 2015, 31(5): 489-492.
MA Qing, LI Shi-jun, LIU Ying, TIAN Ke-chen, TANG Guang-peng, WANG Ding-ming. Etiologic detection and analysis of a human Streptococcus suis infection in Guizhou Province, China. Chinese Journal of Zoonoses, 2015, 31(5): 489-492.
[1] Feng C, Tan MZ, Chen ZH, et al. Clinical features and outcome of infection of type 2 Streptococcus suis in human[J]. J Sichuan Univ, 2007, 38(5): 874. (in Chinese) 冯萃,谭明珍,陈志海,等. 猪链球菌2型感染的临床特征和治疗效果分析[J]. 四川大学学报, 2007, 38(5) : 874. [2] Yang HF, Zhu FC, Shi ZY, et al. Analysis on pathogenic feature of syndrome infected by s.suis from human and swine[J].Chin J Zoonoses. 2001, 17(6): 92-93. (in Chinese) 杨华富,朱凤才,史志扬. 人-猪链球菌感染性综合征的病原特征分析[J]. 中国人兽共患病杂志,2001, 17(6): 92-93. [3] Zhu XP, Zu RQ, Chen ZH, et al. Analysis on features of dead cases with human Streptococcus suis infections[J]. Chin J Epidemiol, 2005, 6(9): 633-635. (in Chinese) 祝小平,祖荣强,陈志海,等. 四川省人感染猪链球菌病死亡病例特征分析[J]. 中华流行学杂志,2005, 6(9): 633-635. [4] Yin JW, Xu W, Gu WP, et al. Pathogenic analysis of the first isolated Streptococcus suis from infected human in Yunnan Province[J]. J Appl Prevent Med, 2012, 18(2): 78-80. (in Chinese) 尹建雯,徐闻,古文鹏,等. 云南省首例人感染猪链球菌病的病原学分析[J]. 应用预防医学, 2012, 18(2): 78-80. [5] Luo HC, Huang J, Zhang WB, et al. Epidemiological investigation of the first isolated Streptococcus suis from infected human in Beihai[J]. J Public Health Prevent Med, 2013, 24(5): 74-75. (in Chinese) 罗华初,黄坚,张万标,等. 北海市首例人感染猪链球菌病的流行病学调查[J]. 公共卫生与预防医学,2013, 24(5): 74-75. [6] Zhu X, Yang TT, Wei JC. Analysis of epidemiological and pathogenic of human Streptococcus suis infection in Dongyang[J]. Mod Prevent Med, 2008, 35(22): 4511-4512. (in Chinese) 朱翔,杨婷婷,韦俊超. 东阳市一起人感染猪链球菌病疫情的流行病学和病原学分析[J]. 现代预防医学, 2008, 35(22): 4511-4512. [7] Chen J, Guo ZN, Wang MZ. et al. Epidemiological investigation of the first isolated Streptococcus suis from infected human in Xiamen[J]. Strait J Prevent Med, 2010, 16(6): 36-37. (in Chinese) 陈健,郭志南,王明斋,等. 厦门市首例人感染猪链球菌病例的流行病学调查[J]. 海峡预防医学杂志, 2010, 16(6): 36-37. [8] Tian KC, Wang DM, Ma Q, et al.Surveillance on Streptococcus suis infection of human and healthy pigs in Guizhou Province[J]. J Appl Prevent Med, 2013, 19( 2): 69-71. (in Chinese) 田克诚,王定明,马青,等. 贵州省猪链球菌病监测结果分析[J]. 应用预防医学, 2013, 19(2): 69-71. [9] Feng ZH, Lin H, Xu YF, et al. Evaluation on effectiveness of API 20 Strep for detection of Streptococcus suis [J]. J Prevent MedInform, 2005, 21(4): 393-394. (in Chinese) 冯泽惠,蔺红,徐跃芳,等. API 20 Strep 生化系统鉴定猪链球菌的效果评价[J]. 预防医学情报杂志,2005, 21(4): 393-394. [10] Vecht U, Wisselink HJ, Jellema ML, et al . Identification of two prot-eins associated with virulence of the Streptococcus suis type2[J]. Infect Immun, 1991, 59: 3156-3162. [11] Zeng QY, Lu CP. Fusion and apoptosis of epithelial cells induced by muramidase released protein of Streptococcus suis type 2[J]. Acta Microbiologica Sinica, 2003, 43(3): 407-411. (in Chinese) 曾巧英, 陆承平. 猪链球菌2型溶菌酶释放蛋白诱导上皮细胞融合和凋亡[J]. 微生物学报,2003, 43(3): 407-411. [12] Norton P, Rolph C, Ward P, et al. Epithelial invasion and cell lysis by virulent strains of Streptococcus suis is enhanced by the presence of suilysin[J]. FEMS. Immunol Med Microbiol, 1999, 26(1): 25-35.
2015, Vol. 31 
