Abstract:The introduction of West Nile Viruses (WNV) to North America in 1999 has accelerated extensive molecular surveillance of this mosquito-borne virus. In this study, in order to comprehensively identify genetic relationship among global circulating WNV strains, genome sequences of 624 isolates in seven decades were phylogenetically analyzed by BioEdit and MEGA software. It was revealed that Lineage-1 strains were distributed widely in most continents among the 4 lineages established worldwide, and that Lineage-1 was further classified into several clades or clusters. In contrast, limited strains of the other lineages were solely isolated in Africa and Europe. Comparing with reference strains of known subtypes, phylogenetic trees were constructed among isolates sharing identical sources including geographic origins and hosts. These results demonstrated the genetic diversity of global WNV strains at genome level. The distinct geography-specific distribution of WNV would facilitate molecular tracking for the evolution and transmission routes of its emerging and re-emerging worldwide in the future.
游丽斌, 王金章, 陈炜, 黄萌, 张拥军. 全球西尼罗病毒毒株基因组种系发生分析[J]. 中国人兽共患病学报, 2015, 31(6): 511-518.
YOU Li-bin, WANG Jin-zhang, CHEN Wei, HUANG Meng, ZHANG Yong-jun. Phylogenetic analysis of global west nile viruses over seven decades. Chinese Journal of Zoonoses, 2015, 31(6): 511-518.
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