Epidemiological characterizes and laboratory investigation of endemic typhus in Hebei Province, 2009-2012
SUN Yin-qi1, DONG Tuo2, 3, JIANG Xia1, WANG Yong2, 4, QIAN Zhen-yu1, LIU Xiao-li1, ZHANG Li-juan2
1.Center for Disease Control and Prevention of Hebei Province, Shijiazhuang 050021, China; 2. National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206, China; 3. School of Public Health, Harbin Medical University, Harbin 150081, China; 4.College of Animal Science &Technology, Shihezi University, Shihezi 832003, China
Abstract：In case of increasing the number of murine typhus cases from hospitals in Hebei Province, a joint investigation was conducted by the National Institute for Communicable Disease Control and Prevention, China CDC and Centers for Disease Control and Prevention of Hebei Province during 2009-2012. Based on the criteria of "Diagnostic criteria for epidemic typhus and endemic typhus (WS215-2008)" issued by Ministry of Health of The People's Republic of China on Feb 28, 2008, 877 clinical diagnostic cases were recruited from Xinji City, Dingzhou City and Qianan City and 101 blood samples from acute stage of illness and 17 blood samples from convalescence of illness were collected respectively, IgM and IgG antibodies against Rickettsia typhi in sera were tested by immunofluorescence assays (IFA). Nested PCR targeted R. typhi groEL gene was performed using acute stage blood clots DNA as temples and phylogenetic analysis was conducted based on the groEL gene. Epidemiological data indicated the highest annual incidence was 2009 with 441 cases and the highest areas were Qianan City with 510 cases. Of the acute phase sera, 72.8% (73/101) were IgM positive and 78.2% (79/101) were IgG positive. A 4-fold seroconversion in the IgG antibody titer was observed in 12 patients (4-fold increase in 7 patients and 4-fold decrease in 5 patients). The 21.7% (22/101) of acute stage blood clot DNA were nested PCR positive. Sequence analysis showed the partial sequence of groEL genes from 17 patients were 100% identity for each other. Compared with the reference stain R. typhi Wilmington (AY191590), only 97.0% of nucleotide homology and 93.9% amino acid homology were noticed and phylogenetic analysis indicated the groEL genes tested in the study were far from other sequences identified in other areas of the world. Here, we concluded that the reemerging murine typhus was confirmed in most areas of Hebei Province, China, and diagnosis and differential diagnosis of the R. typhi infection should be emphasized in hospitals.
孙印旗, 董妥, 姜霞, 王勇, 钱振宇, 刘晓丽, 张丽娟. 2009-2012年河北鼠型斑疹伤寒流行概况及实验室调查分析[J]. 中国人兽共患病学报, 2015, 31(6): 552-555.
SUN Yin-qi, DONG Tuo, JIANG Xia, WANG Yong, QIAN Zhen-yu, LIU Xiao-li, ZHANG Li-juan. Epidemiological characterizes and laboratory investigation of endemic typhus in Hebei Province, 2009-2012. Chinese Journal of Zoonoses, 2015, 31(6): 552-555.
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