Gene mutation of coxsackievirus A4 VP1 from Shenzhen, China, 2014
YAO Xiang-jie1, HE Ya-qing1, CAI Chun-lin2, ZHUO Fei2, YANG Gui-qing2
1.Laboratory of Infectious Diseases and Surveillance,Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China; 2.Luohu Center for Disease Control and Prevention, Shenzhen 518020, China
Abstract：We identified the pathogen causing an outbreak of herpangina in Shenzhen in 2014. Phylogenic analysis was carried out on VP1 genetic region of coxsackievirus A4 isolated from this outbreak. Eight throat swab specimens were collected from patients during the outbreak. The RNA was extracted from the virus and real-time RT-PCR method was used to test virus such as human enterovirus71, coxsackievirus Al6, coxsackievirus A4, coxsackievirus A6, and coxsackievirus A10. Complete VP1 gene of CVA4 identified in the outbreak was amplified by RT-PCR technique. Phylogenetic trees based on entire and partial VP1 sequences were constructed among CVA4 gene and others published in GenBank. It showed that CVA4 with GIB type was the pathogen in this outbreak. Data from homological comparisons indicated 4 CVA4 strains amplified in this outbreak had the highest nucleotide acid identity with the isolates from Yunnan in 2004 (AB268278), Shenzhen in 2009 (HQ728260), and Taiwan in 2008 (AB571570). The homologies of nucleotide sequences among them were 94.1%- 94.8%, which has the lowest identity (84.3%) with the prototype strain (HIGHPOINT strain). However, the 4 CVA4 strains amplified in this outbreak had the highest amino acid identity (99.3%) with the isolates from Taiwan in 2006 (AB571563), Jilin in 2009 (JQ715709), and the lowest amino acid identity (97.1%) with the isolates from Shandong in 2006 (GQ253375). Comparing with the CVA4 isolate from Shenzhen in 2009, 6 amino mutation were found in the four CVA4 strain (N22S,T34A, N63S, A165D, T200A and V126A ). Result of the analysis on phylogenetic tree showed that the 4 CVA4 stains were still belong to GIB subtype, however, it is obviously different with other stains in GIB group gained in other place in different time. In conclusion, the four CVA4 stains identified in Shenzhen, 2014 belongs to GIB subtype, which were found to have great difference on VP1 region.
姚相杰, 何雅青, 蔡春林, 卓菲, 杨贵清. 深圳地区2014年柯萨奇病毒A组4型VP1区基因特征分析[J]. 中国人兽共患病学报, 2015, 31(6): 565-568.
YAO Xiang-jie, HE Ya-qing, CAI Chun-lin, ZHUO Fei, YANG Gui-qing. Gene mutation of coxsackievirus A4 VP1 from Shenzhen, China, 2014. Chinese Journal of Zoonoses, 2015, 31(6): 565-568.
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2015, Vol. 31 
