1.Fujian International Travel Healthcare Center, Fuzhou 350001, China; 2.Fujian Medical University, Fuzhou 350004, China; 3.Fujian Center for Disease Control and Prevention, Fuzhou 350001, China
Abstract：In this study, we aimed to establish method for identifying Sarcophaginae by DNA-barcoding and to solve the problem that Sarcophaginae female species could not be identified by morphology. We amplified and analyzed the sequences of these samples in Fujian frontier such as Helicophagella melanura, Parasarcophaga misera, Parasarcophaga sericea, Boettcherisca peregrine, Parasarcophaga brevicornis, Parasarcophaga dux, Parasarcophaga similis, Parasarcophaga albiceps, Parasarcophaga Pingi, and Seniorwhitea reciproca. Secondly, we aligned these sequences with 88 Sarcophaginae species loaded from BOLD. Thirdly, we estimated the genetic distances and built system evolutionary tree. The result of amplification with 5 samples showed that length of the obtained COI sequences were 658 bp. And the result of alignment on BOLD line showed that index of similarity of the same species was above 99%. The result of calculation genetic distances showed that intraspecific difference was 0-0.015 4 and interspecific difference was 0.024 9-0.153 8. Phylogenetic tree showed that the same Sarcophaginae species stayed together on value of Bootstrap 1000. It came to a conclusion that DNA-barcoding can be the important method to supplement of morphologically identifying Sarcophaginae.
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2015, Vol. 31 
